HPLC-ELSD法测定龙眼肉中果糖、葡萄糖、蔗糖的含量

Determination of Fructose,Glucose and Sucrose in Arillus longan by HPLC-ELSD

目的：建立龙眼肉药材中果糖、葡萄糖、蔗糖的HPLC—ELSD含测方法，为科学评价龙眼肉质量提供依据。方法：采用Phenomenex Luna NH2柱（4．6mi／1×250into，5μm），乙腈-水（80：20）为流动相。结果：果糖、葡萄糖、蔗糖均能很好分离，线性范围和相关系数分别为：1．532～7．660μg（r=0．9998）、1．554—7．770μg（r=0．9999）及2．032～10．160μg（r=0．9998），平均回收率（n=9）分别为101．26％（RSD=1．87％）、101．39％（RSD=1．11％）及102．91％（RSD=1．19％）。结论：该方法简便、准确，分离效果好，无干扰，适用于该药材中糖类成分含量测定。

Objective： To establish an HPLC-ELSD method for the determination of fructose, glucose and sucrose in Arillus longan,and to investigate their contents in different places. Methods： The sample were separated on a Phenomenex Luna NH2 column （4. 6 mm× 250 mm,5μm） eluted with acetonitrile-water （80： 20） as mobile phase. Results： The methodological studies showed that the Fruc- tose, Glucose and Sucrose were separated. A good linearity was obtained with the correlation coefficients ranged from 1. 532 to 7. 660 μg （ r = 0. 999 8 ）, from 1. 554 to 7. 770 μg（ r = 0. 999 9） and from 2. 032 to 10. 160μg （ r = 0. 999 8）, respectively. The average recov- eries of the three components（n =9） were 101.26% （RSD = 1.87% ） ,101.39% （ RSD = 1. 11% ） and 102. 91% （ RSD = 1.19% ）, respectively. Conclusion：It was proved that the method with HPLC-ELSD was reliable, simple and well separated. It can be used as determination of saccharides in Arillus longan.