摘要
目的：建立羚竺散中黄连、前胡、甘草的薄层色谱方法及黄连中盐酸小檗碱的高效液相含量测定的方法。方法：采用反相高效液相色谱法，选用Diamonsil C18色谱柱，流动相为乙腈-0．1％磷酸溶液（每100mL中加入0．1g十二烷基磺酸钠）（48：52），流速为1．0mL·min^-1，检测波长为265nm。结果：盐酸小檗碱在0．00406～0．04060mg·L^-1范围内线性关系良好（r=0．9998），平均回收率为99．2％，RSD为1．36％（n=6）。结论：该方法操作简便，结果准确，可用于羚竺散的质量控制。
Objective： To identify Rhizoma Coptidis, Radix Peucedani and Radix Glycyrrhizae by TLC method and to determine Berberine in Lingzhu Powder by RP-HPLC. Method： The Diamonsil C18 column was used. The mobile phase was acetonitrile-0. 1% phosphoric acid solution（0.1 g of sodium dodecylsulfate was dissolved in 100 mL of water） （48： 52） with the flow rate of 1.0 mL·min^-1. The detection wavelength was 265 nm. Results ： The calibration curves of Berberine was linear in the range of 0. 004 06 - 0. 040 60 mg·L^-1 （r =0. 999 8）. The average recovery was 99.2% with RSD 1.36% （n =6）. Conclusion： The method was simple,accurate and reliable. It can be used in the quality control of crude drug.
出处
《中国药品标准》
CAS
2011年第1期67-69,共3页
Drug Standards of China
