谷氨酸高产菌TG-866的选育及发酵罐试验

SELECTION OF GLUTAMATE-PRODUCING STRAIN TG-866 AND FERMENTATION EXPERIMENT IN 500L FERMENTOR

以TG—3菌为亲株,经紫外线、硫酸二乙酯的复合诱变,定向选育抗单氟乙酸、耐高糖、耐高谷氨酸、以谷氨酸为唯一碳源不长和以琥珀酸为唯一碳源生长良好的突变株,选育出TG—866谷氨酸高产菌.在实验室的摇瓶试验中,连续4批达到平均产酸8.37%,平均转化率55.1%。在500L发酵罐上以淀粉水解糖为原料,取得了连续5批产酸7.5％以上,转化率50％以上的成绩。

TG≡866 Was breeded from TG-3 as parent mutagenized by ultraviolet and DES. The mutant has characteristics of high glucose resistance, high glutamate, resistance, monofluorine acetate resistance, litter grouth with only glutamate as C source and good grouth with only sucinate as C source. In 4 continuous batchs shaker experiments, average yield of gluta mate and convertion rate of glucose were 8.37% and 55.1%, respectively. In 500L fermentor, the result was achieved that the yield of glntamate was over 7.5% and the convertion rate of glucose was over 50% in 5 continuous bakchs fermentation with starch hydrolyzate sugar.