利用高糖高脂饮食建立胰岛素抵抗大鼠模型

Establishment of Insulin Resistance Model with High-sugar and High-fat Diet in Rats

目的:通过给予Wistar大鼠喂养高糖高脂饮食,探索诱发建立胰岛素抵抗(IR)动物模型的方法。方法:24只Wistar大鼠随机分为普通饲料组和高糖高脂饲料配方组,喂饲12周后采血,生化及酶联免疫分析检测血糖、血脂及胰岛素水平,观察其对血清胰岛素抵抗的影响。结果:与对照组比较,模型组大鼠血低密度脂蛋白(LDL-C)、总胆固醇(TC)、甘油三酯(TG2)、空腹血糖(FPG)明显升高,且具有统计学意义(P<0.05),并且两组之间比较,空腹血胰岛素水平(FINs)、胰岛素敏感指数(ISI)、胰岛素抵抗指数(HOMO-IR)亦有明显差异,ISI明显下降,而HOMO-IR明显上升,且具有统计学意义(P<0.05)。结论:通过给Wistar大鼠喂养高糖高脂饮食能够诱发稳定的高糖高脂大鼠模型,可以初步建立胰岛素抵抗伴有胰岛素分泌不足的动物模型,而不只是单一的胰岛素抵抗动物模型。这一动物模型符合人类胰岛素抵抗发生的规律,是较为理想的动物模型。

Objective To explore methods whether the Insulin Resistance model can be established through feeding high-sugar and high-fat diet in Wistar rats. Methods 24 Wistar rats were randomly divided into control group （feed with common diets） and model group （feed with high-fat and high-sugar diets）. After 12 weeks, rat＇s blood was collected, and fasting plasma glucose, fasting plasma lipids and insulin levels were detected by biochemistry or ELISA technique to evaluate the effects of different diets on insulin sensitivity index （ISI） and insulin resistance index （HOMO-IR）. Results The serum low density lipoprotein （LDL-C）, total cholesterol （TC）, triglyceride （TG2）, fasting plasma glucose （FPG） of the model group was significantly increased, comparing with the control group（P〈0.05）. Fasting insulin levels （FINs）, insulin sensitivity index （ISI）, insulin resistance index （HOMO-IR） were also significant differences between two groups, such as IS7 decreased obviously （P〈0.05）, while the HOMO-IR increased significantly （P〈0.05）, comparing with the control group. Conclusion We could obtain a stable high glucose and high fat rat model through feeding Wistar rat with high-sugar and high-fat established the insulin resistance animal model with hypoinsulinism, rather than resistance. This animal model of insulin resistance occurred in line with the law diet, and may be able to initially a single animal model of insulin of humanity, it is an ideal animal model.