龙葵多糖对H_(22)荷瘤小鼠红细胞膜功能的影响

Effects of solanum nigrum polysaccharide on functioning of erythrocyte membrane of H_(22) tumor-bearing mice

研究龙葵多糖对H22荷瘤小鼠红细胞膜功能的影响.采用DPH荧光探针标记荧光分光光度计测定红细胞膜的流动性,比色法测定红细胞膜表面膜封闭度、SA、SOD的含量.龙葵多糖各剂量组都能够降低H22荷瘤小鼠红细胞膜的微黏度,提高细胞膜脂流动性,提高红细胞膜重新封闭的能力,升高荷瘤小鼠红细胞膜唾液酸及超氧化物歧化酶的含量.龙葵多糖可能通过改善和恢复H22荷瘤小鼠红细胞膜功能而达到抗肿瘤的作用.

To study the effect of solanum nigrum polysaccharide on the functioning of red cell membrane in H22 tumor-bearing mice.The membrane fluidity of red cells was measured by fluorescence spectrophotometer with DPH fluorescence probe as a marker;the amount of sialic acid and SOD on the surface of red cell membrane and the sealability of erythrocyte membrane were measured using colorimetric analysis assay.Solanum nigrum polysaccharide reduced the membrane microviscosity,at the same time,enhanced the membrane fluidity of red cells in H22 tumor-bearing mice,while increasing the amount of sialic acid and SOD in the membrane of red cells in these mice and enhancing the ability of the membrane of their red cells to reseal.The anti-tumor effect of solanum nigrum polysaccharide on H22 tumor-bearing mice may be due to its ability to improve and restore the functions of the erythrocyte membrane.