摘要
从刺参(Apostichopus japonicus)养殖池塘环境中驯化分离筛选到1株低温有机物和氨氮降解菌株DB11。根据其形态特征、生理生化特性以及16S rDNA序列分析,将该菌株鉴定为马氏副球菌(Paracoccus marcusii)。经驯化,该菌株以刺参饵料为唯一营养源,低温(15℃)、低接种量(〈5×10-3)条件下能同时高效降解饵料中的有机物和氨态氮,5 d时间内对富集培养基中COD和NH4+-N的降解率分别达50%和98%。进一步研究其降解特性表明,菌株生长适温15~30℃,生长适宜pH值为7~10,降解刺参饵料中COD和NH4+-N的最适温度条件为15~20℃、最适pH条件为8.0~8.5;在最适降解条件下、接种量为5×10-3时,对10~20 g/L高质量浓度的刺参饵料液中COD降解效果显著,3 d时间去除率达56.9%~65.7%,对1~20 g/L质量浓度的刺参饵料液中NH4+-N 3 d时间的去除率达91.7%~99.9%。
A low-temperature bacterium named DB11 was isolated from sea cucumber outdoor culturing ponds with high degradation of organic-pollutants and ammonia-nitrogen.Physiological and biochemical tests and the 16S rDNA sequence similarity analysis indicated that strain DB11 was similar to Paracoccus marcusii.When inoculated into the sea cucumber-feed-enrichment-medium by less than 5×10-3,strain DB11 was able to utilize 50% of COD and 98% of NH+4-N within five days at 15 ℃.Further degradation studies showed that the appropriate range of temperature and pH value for growth of strain DB11 was 15~30 ℃ and pH 7~10,respectively,while the optimal temperature and pH value conditions for degradation of sea cucumber feed was 15~20 ℃ and pH 8.0~8.5,respectively.Strain DB11 could decompose sea cucumber feed concentration of 10~20 g/L with 56.9%~65.7% of COD degradation rate,and feed concentration of 1~20 g/L with 91.7%~99.9% of NH+4-N degradation rate,respectively,within 3 days after inoculated by 5×10-3.
出处
《中国海洋大学学报(自然科学版)》
CAS
CSCD
北大核心
2011年第3期24-30,共7页
Periodical of Ocean University of China
基金
国家十一五科技支撑计划课题(2006BAD09A01)
国家十一五支撑计划项目(2006BAD09A06)
国家高技术研究发展计划项目(2006AA10Z409)
山东省自然科学杰出青年基金项目(JQ201009)资助
关键词
低温菌
有机物
氨氮
分离鉴定
降解特性
low-temperature bacterium
organic-pollutants
ammonia-nitrogen
isolation and identification
degrading characteristics