密鳞牡蛎的人工繁育

Techniques of Artificial Breeding of the Oyster Ostrea denselamellosa

研究密鳞牡蛎（Ostrea denselamellosa）的亲体培育和苗种培育技术;研究盐度、温度、密度和饵料等因子对浮游幼虫生长及存活的影响,首次建立密鳞牡蛎的人工繁育技术。结果表明,排出体外的D形幼体在水温23~25℃,经约28 d出现眼点,进入附着变态阶段;幼虫培育的最佳盐度为30;在24~32℃,幼虫生长速度与温度呈正比,成活率与温度成反比;在培育密度为1,2,3和4/mL 4组中,1/mL组生长最快,但各组间存活率无显著差异;湛江等鞭金藻,50%湛江等鞭金藻＋50%扁藻,50%湛江等鞭金藻＋50%小球藻和小球藻4种饵料投喂组中,50%湛江等鞭金藻＋50%扁藻组的生长显著高于其他组;不同附着基的附苗率依次为：扇贝壳〉波纹板〉筛绢〉网片〉聚乙烯薄膜。

Broodstock conditioning and larval rearing were for the first time investigated in the oyster Ostrea denselamellosa.The effects of salinity,water temperature,density and diets on growth and survival rates of O.denselamellosa larvae were also examined.After larval rearing of about 28 days,the larvae developed to the stage of metamorphosis characterized by the appearance of eye spot in 28~30 psu at 23~25 ℃.As the temperature changed from 24 ℃ through 32 ℃,the growth rate of the larvae was positively correlated to temperature,while the survival rate negatively correlated.For the four experimental groups with stocking densities of 1,2,3 and 4 larvae mL-1,larvae reared at the lowest density had the largest mean size,but there is no significant difference in survival rate.Compared with two monoalgal diets of Isochrysis zhanjiangensis and Platymonas subcordiformis and a mixed diet of I.zhanjiangensis and Chlorella vulgaris,the highest growth rate was achieved with the mixed diet of I.zhanjiangensis and P.subcordiformis.The setting rates of the oyster larvae varied depending on the type of substrate used.The scallop shell had the highest setting rate of larvae,followed by plastic sheet,screen mesh,monofilament nylon and plastic film.