高尔基蛋白73单克隆抗体在肝细胞肝癌诊断中的价值

Value of Golgi Protein 73 Monoclonal Antibody in Diagnosis of Hepatocellular Carcinoma

目的自制高尔基蛋白73(GP73)单克隆抗体并检测其诊断肝细胞肝癌(HCC)的敏感性和特异性。方法自行制备GP73单克隆抗体,作为一抗用Western blot方法检测59例HCC患者和31名健康对照者血清中GP73蛋白的表达情况,并对结果进行半定量分析,确定对照组人群的正常基线水平。将此结果同多克隆抗体的检测结果及甲胎蛋白(AFP)的诊断结果进行比较。结果使用单克隆抗体检测健康人群的血清GP73水平为1.2(0.9-1.7)相对单位(RU),HCC患者血清GP73水平为5.7(2.5-7.8)RU,显著高于对照组水平(P<0.001);多克隆抗体检测结果显示,HCC患者血清GP73水平与对照组相比[7.8(3.0-12.4)RU比1.1(1.0-2.0)RU]亦显著增高(P<0.001)。GP73单抗诊断HCC的敏感性和特异性分别为84.7%和93.5%,对比多抗的敏感性(78.0%)和特异性(93.5%),前者敏感性有所增高;同组患者采用AFP诊断HCC的敏感性和特异性分别为67.8%和74.2%,均低于GP73单抗。Logistic回归分析显示,GP73单抗的优势比(OR)为7.18,而GP73多抗的OR为1.51。结论单克隆抗体可以有效地检测肝癌患者血清GP73水平,比AFP具有更高的敏感性和特异性;可能优于目前使用的GP73多克隆抗体的检测。这一结果为用自制单抗进一步开发酶联免疫方法检测奠定了基础。

Objective To explore the sensitivity and specificity of Golgi protein 73（GP73） monoclonal antibody in the diagnosis of hepatocellular carcinoma（HCC）.Methods Self-prepared GP73 monoclonal antibody was used as the primary antibody for detecting the serum GP73 levels in healthy controls（n=31） and HCC patients（n=59）.The baseline level of the healthy controls was determined by semiquantitative analysis.The results were compared with those from GP73 polyclonal antibody and alpha-fetoprotein（AFP）.Results The GP73 level of healthy controls was 1.2（0.9-1.7） relative unit（RU）,which was significantly lower than that of HCC patients [5.7（2.5-7.8） RU]（P0.001） with monoclonal antibody.Using polyclonal antibody,the GP73 level of HCC patients was also significantly higher than healthy controls [7.8（3.0-12.4） RU vs.1.1（1.0-2.0） RU,P0.001].The sensitivity and specificity of GP73 monoclonal antibody in diagnosis of HCC were 84.7% and 93.5%;on the contrary,those of GP73 polyclonal antibody were 78.0% and 93.5%,respectively.The sensitivity and specificity of AFP（67.8% and 74.2%,respectively） in the HCC patients were markedly lower than those of GP73.Logistic regression analysis showed that the odds ratio（OR） of GP73 monoclonal antibody was 7.18 and that of GP73 polyclonal antibody was 1.51.Conclusions Our self-prepared monoclonal antibody can effectively detect GP73 serum level in HCC patients,and has higher sensitivity and specificity than AFP.It may be superior to the currently used GP73 polyclonal antibody.The results lay the foundation for the further development of ELISA methods by using this monoclonal antibody.