鱼肝油酸钠对回肠新膀胱粘液分泌的影响

Influence of soduim morrhuate on mucus production of neo-bladder of rats

[目的]了解鱼肝油酸钠和酒精对回肠新膀胱黏膜和杯状细胞分泌功能的影响。[方法]SD大鼠24只,体重250 g,随机分为生理盐水对照组(A组)、75%酒精组(B组)、鱼肝油酸钠组(C组),每组8只。大鼠回肠新膀胱模型建立后,分别第8、11、14天新膀胱灌药,每次灌药后72 h留取尿液,测定尿液蛋白浓度;第17天处死大鼠,取新膀胱标本,光镜和电镜观察形态变化,免疫组化方法检测NF-кB表达,以评估新膀胱黏膜状况及粘液分泌情况。[结果]与A组新膀胱尿液中蛋白浓度(4.48±0.56)mg/mL相比,B组(1.21±0.07)mg/mL、C组(1.69±0.36)mg/mL均显著降低(P<0.05);光镜下,A组新膀胱肠绒毛整齐,黏膜完整,肌层未见损伤,杯状细胞所占比例:(0.31±0.03)/HP;B组新膀胱肠绒毛结构紊乱,黏膜不完整,肌层有不同程度损伤,杯状细胞所占比例(0.08±0.04)/HP,低于A组。C组新膀胱肠绒毛无明显异常,黏膜及肌层无明显损伤,杯状细胞所占比例(0.12±0.05)/HP,较A组降低;核因子NF-кB阳性表达细胞数为:A组(27.88±7.18)、B组(7.50±2.20)、C组(11.88±2.61),后二组均显著降低(P<0.05);电镜下,A组新膀胱肠黏膜微绒毛整齐,细胞器正常;B组无微绒毛结构,大量细胞液化坏死,颗粒释放;C组微绒毛变短、稀疏或缺失,细胞器基本正常。[结论]鱼肝油酸钠较75%酒精更适合应用于术后灌注回肠新膀胱。

[Objective] To investigate the effects of morrhate sodium and 75% alcohol on the intestinal mucosa and goblet cells of neo-bladder in rat model.[Methods] SD rats of 24 weighing about 250 grams were randomly divided into saline（A）,morrhate sodium（B）,and 75% alcohol（C） groups.The drugs were injected to the neo-bladders at the 8th,11th,and 14th day after the operation of neo-bladder reformation from ileum,and the urine in neo-bladder were collected at 72 hours following drug injection.The rats were terminated at the 17th day.The neo-bladder tissues were observed under the light microscopy and electron microscopy,and NF-кB expression in the tissues were detected by immunohistochemisty for comparing the degree of injury.[Results] Protein concentrations of group A were（4.48±0.56） mg/mL,group B were（1.21±0.07） mg/mL,and group C were（1.69±0.36）mg/mL,respectively（P0.05）.Microscopy,in group A,the intestinal mucosa was complete and intestinal villus was regular,muscular layer injury was not clear,the proportion of goblet cells was（0.31±0.03）/HP,in group B,the intestinal mucosa was not complete,and intestinal villus was disorder,the