荧光磁性纳米粒子标记间充质干细胞靶向胃癌研究

Study of Fluorescent Supermagnetic Nanoparticles Labeled Mesenchymal Stem Cells Tropism for Gastric Cancer

目的:探索一种高效的早期胃癌诊断体系。方法:荧光磁性纳米粒子与间充质干细胞共培养,不同时间点检测干细胞存活率。利用荧光显微镜、普鲁士蓝染色、透射电子显微镜等方法观测干细胞被标记情况;建立裸鼠的胃癌模型,并将标记后的干细胞尾静脉注射入裸鼠体内,14d后用动物成像仪和核磁共振成像仪检测。结果:荧光磁性纳米粒子在低于100μg/mL浓度下对间充质干细胞没有毒性作用,荧光磁性纳米粒子与干细胞共培养6h后,荧光显微镜、普鲁士蓝染色、透射电子显微镜检测结果显示粒子被内吞后定位于细胞质中,动物实验显示通过检测肿瘤部位的荧光信号和核磁信号,被标记的干细胞能够准确定位到胃癌的发生部位。结论:这种双模式检测体系为胃癌的早期诊断与治疗提供一种新的方法。

Objective：To establish a system for efficient gastric cancer early diagnosis.Method：Marrow mesenchymal stem cells（MSCs）were co-cultured with FSMNPs and then tested their survival rate in different periods.Fluorescence microscope,Prussian blue staining,transmission electron microscopy were used to observe labeled MSCs;nude mouse models loaded with gastric cancer were established and then labeled MSCs were intravenously injected into these mice.The signals were detected by using animal imaging and magnetic resonance imaging after 14 days.Result：The prepared FSMNPs displayed almost no toxicity to MSCs below the concentration of 100μg/mL.,MSCs were co-cultured with FSMNPs in 6 hours and then fluorescence microscope,Prussian blue staining,transmission electron microscopy imaging showed FSMNPs located in cytoplasm by endocytosis.Animal experiments displayed FSMNPs labeled MSCs could target and home to gastric cancer through testing fluorescenct and MRI singal at the tumour site.Conclusion：This dual-mode detection system would provide a new method for gastric cancer early diagnosis and therapy.