CDKN2A基因失活对喉癌的影响及其临床意义

Inactivation of CDKN2A gene in larynx cancer patients and its clinical significance

目的研究细胞周期依赖性激酶抑制基因(CDKN2A)失活对喉癌的影响及其临床意义。方法应用半定量反转录聚合酶链反应(RT-PCR)和免疫印迹分析法检测喉癌手术切除组织68例,非肿瘤喉部组织68例,观察CDKN2A基因失活与喉癌发生发展的关系。结果 (1)喉癌组织CDKN2A mRNA表达明显少于非肿瘤喉组织(P<0.05);(2)喉癌组织中CDKN2A蛋白的表达明显低于非肿瘤喉组织,差异有统计学意义(两者的低表达率为80.9%、47.1%,过低表达率为52.9%、22.1%,P<0.05),并与病理分型无关;(3)Western blot电泳结果半定量分析显示,喉癌组织CDKN2A蛋白的表达水平明显低于非肿瘤喉组织(t=3.246,P<0.05)。结论CDKN2A基因的变异及其编码蛋白质的失活与喉癌的发生发展明显相关。

Objective To study the inactivation of cyclindependent kinase inhibitor 2A（ CDKN 2A） gene and to analyze its clinical significance in larynx cancer. Methods The resected tissues of larynx cancer surgery and non - tumor larynx tissue were collected from 68 patients. The expression of CDKN2A was assayed by the semiquantitative counter reverse transcription polymerase chain reaction （RT -PCR）. The protein expression of CDKN2A was determined by Western blot analysis. The relationship between CDKN2A expression and the occurrence and development of larynx cancer was analyzed. Results （ 1 ） The CDKN2A mRNA expression in laryngeal carcinoma was significantly less than that in non -tumor laryngeal tissues （P 〈 0.05 ）. （2）The expression of CDKN2A protein in laryngeal carcinoma was significantly lower than that in non - tumor laryngeal tissues （ the low expression rates were 80.9% and 47. 1% , respectively, and the lower expression rates were 52.9% and 22.1% , respectively , P 〈 0. 05 ）. The expression was not related to pathological types. （3）Western blot analysis showed that the CDKN2A protein expression in laryngeal carcinoma was significantly lower than that in non - tumor laryngeal tissues （ t = 3. 246, P 〈 0.05 ）. Conclusions The CDKN2A gene mutation and inactivation of its encoded protein are related to the development of laryngeal cancer significantly.