环介导等温扩增技术的应用

The application of loop-mediated isothermal amplification(LAMP)

环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是针对靶基因的6个区域设计4种特异引物,利用一种链置换型DNA聚合酶(Bst DNA polymerase)在恒温65℃左右保温几十分钟快速进行核酸扩增的方法。由于其具有快速、简便、特异性好、灵敏度高、成本低等优点,已经应用于临床诊断、食品卫生检疫及基因芯片的开发,特别是在细菌和病毒的检测方面有重要意义。

Loop-mediated isothermal amplification（LAMP） is a novel nucleic acid amplification method. The LAMP method employs a DNA polymerase （Bst DNA polymerase） with strand displacement activity and a set of 4 different primers specifically designed to recognize 6 distinct regions on the target DNA. The amplification reacts under 65℃ and the products are stem-loop DNA structures with multiple loops. Because LAMP recognizes the target by 6 distinct sequences initially, it is expected to amplify the target sequence with high selectivity. The reverse-transcription loop-mediated isothermal amplification （RT-LAMP） technology has been evaluated for the detection of RNA. The reaction mixture is the same with LAMP except for the adding of a reverse transcripatase. The purpose of this study is to review the current knowledge of this field as well as future research approaches from an applied perspective.