脂多糖对奶牛子宫内膜上皮细胞毒性作用研究

Toxic Effect of LPS on Bovine Endometrial Epithelial Cell

本试验通过传代培养奶牛子宫内膜上皮细胞,以0.1μg/mL、1μg/mL、10μg/mL、50μg/mL、100μg/mL、500μg/mL、1000μg/mL 7个LPS浓度梯度刺激细胞,分别于刺激后12h、24h、36h 3个时间段做MTT细胞毒检测,同时进行细胞形态学观察,研究LPS对子宫内膜上皮细胞的毒性作用。结果发现:0.1μg/mL、1μg/mLLPS组细胞形态学无明显改变;10μg/mL、50μg/mL、100g/mL LPS组细胞形态学与对照组相比有明显改变,500μg/mL、1000μg/mL LPS组细胞发生裂解、死亡。MTT试验结果表明LPS对细胞的抑制作用呈浓度与时间依赖关系,随着LPS浓度的增加和刺激时间的延长,细胞抑制率明显增加,细胞活力明显降低。

The bovine endometrial epithelial cells subcultured were stimulated by LPS at 0.1μg/mL,1μg/mL,10μg/mL,50μg/mL, 100μg/mL,500μg/mL and 1000μg/mL seven different concentrations and the toxic effect of LPS was test by MTT methods at the time of 12h,24h,36h,meanwhile,the cell morphology was observed.The results suggested that the cell morphology was not changed obviously in 0.1μg/mL,1μg/mL LPS group compared with the control group,but it was opposite in 10μg/mL,50μg/mL and 100μg/mL LPS group.The cells were clearaged and dead in 500μg/mL and 1000μg/mL LPS group.MTT test showed that the depressant effect of LPS on the cell was in concentration and time dependent manner.With the increase of LPS dosage and extension of stimulated time,the inhibition ratio increased and cell vitality decreased.