摘要
以94头荷斯坦牛为试验材料,应用PCR-SSCP技术研究Leptin基因外显子2多态性与繁殖力的相关性。在Leptin基因外显子2的E2FB区上,经分析存在影响奶牛繁殖的多态位点,测序首次发现102nt处T突变为C。在平均产犊间隔上,AA与AT差异极显著(P<0.01);AT与TT差异极显著(P<0.01);A为优势等位基因,可作为荷斯坦牛育种中繁殖力的分子辅助标记。
PCR-SSCP technology were employed to analyze the correlation between Leptin gene＇s polymorphism and fertility traits in 94 Holstein cows.The polymorphism（T/C）in the Locus 102bp affecting the cows＇ fertility was firstly detected in the exon 2 area （E2FB）of Leptin gene,after Sequencing.For the average parity interval,there were significant differences between genotype AA and genotype AT（P〈0.01）,between genotype AT and genotype TT（P〈0.01）.Genotype A was dominant allele,which could be applied for the molecular genetic markers in fertility of Holstein cows.
出处
《中国奶牛》
2011年第6期4-7,共4页
China Dairy Cattle
基金
现代农业(奶牛)产业技术体系建设项目(nycytx-0106)
