活性碳、微量元素、大量元素对烟草花药培养的影响

Effect of Activated Carbon,Microelement ,Macroelement on Anther Culture in Tobacco

以Ｈ（１９６７）为基本培养基、烟草花药为材料，研究了烟草花药培养中影响出苗率的因素。结果是：提高原Ｈ配方中微量元素的倍数或降低大量元素的倍数有利于烟草花培出苗；活性碳的促进作用与培养基中大量元素和微量元素之间的比例有关；活性碳的浓度以１．０％～２．０％出苗效果最好；活性碳经水洗后促进效果减弱，活性碳提取液有促进出苗的作用；活性碳杂质中Ｆｅ和Ｚｎ的作用是主要的。结果说明：活性碳在烟草花药培养中的作用主要是通过其杂质中Ｆｅ和Ｚｎ等增加了培养基中微量元素的含量，使培养基中微量元素与大量元素的比例趋于平衡，促进胚状体的形成和发育，提高出苗率。

To raise the microelement or reduce the macroelement in H deriction was favourable of the rate of emergence in the anther culture; The promotion of AC was related to the promotion between microelement and macroelement in the culture medium; The highest rate of emergence should take the concentration 1.0%～ 2.0% of AC; The promotion was abated after washed AC, but the liquid extracted from AC promoted the rate of emergence, So we think Fe, Zn in AC are important in the anther culture, they keep the balance between microelement and microdement in the culture medium H(1967) and raise the rate of emergence.