用PDS-1000/He型基因枪将外源基因质粒pZFX_2导入小麦细胞的研究

Study on introduction of foreign NPT Ⅱ gene into cells of wheat

小麦是世界上分布广、种植面积大的粮食作物，选育高产、优质、抗病等优良性状的小麦新品种具有重要的意义。近年来，随着植物遗传转化技术的不断完善，小麦的遗传转化也取得较大突破，先后采用基因枪法、花粉介导法、ＰＥＧ法和激光穿刺法等，将ＮＰＴⅡ、Ｂａｒ、ＧＵＳ...

Foreign NPT Ⅱ gene was introduced into calli and mature embryos of wheat strains 922 267, 942 114 and 953 1575 by using PDS 1000/He particle delivery system. Plasmid pZFX 2 contains the NPT Ⅱ (neomycin phosphotransferase) and glycolate oxidase gene which can increase the wheat plant resistance to insects. After selection culture based on MS medium containing 100 μg/mL Kanamycin, resistant calli were selected. Green plants were regenerated from part of resistant calli. Through this test, we found that the optimum pressure of bombarding calli was 1 100 psi. Using this pressure, more resistant calli were obtained. Mature embryos were bombared at pressure of 1 300 psi. It is worth to notice that the green plants derived from transformed mature embryos were easier than those from transformed calli.