新疆石榴皮多酚对实验性结肠炎的药效学研究

Drug action of Pomegranate Peel Polyphenol on Experimental Colonitis

目的:研究石榴皮多酚提取物(PPPE)对实验性结肠炎的功效及其机制。方法:三硝基苯磺酸(TNBS)灌肠建立KM小鼠溃疡性结肠炎模型,i.g给药15 d,观察评价疾病活动指数(DAI)、结肠黏膜损伤指数(CMDI)、肠重指数(IWI),脾脏指数(SI)、胸腺指数(THI)、结肠、脾脏组织病理学变化,检测血清溶血素(SH)、结肠组织白介素IL-4、IL-17水平。结果:经PPPE干预后结肠病理状况明显改善,脾脏白髓萎缩状况改善,DAI、CMDI、IWI降低,SH含量显著升高,IL-17水平降低。结论:PPPE能有效改善结肠炎症,可能与提高小鼠体液免疫功能、减少炎性细胞因子水平等有关。

OBJECTIVE： To observe the effects of Pomegranate peel polyphenol （PPPE） on ulcerative colitis in mouse and approach it＇s mechanism of immunoloregulation. METHODS：The KM mouse were divided into four groups ： mormal, model, PPPE, SASP group, drug or normal saline were administered intragastrically for 15 days, then trinitrobenzene sulfonic acid （TNBS） were used to induce ulcerative colitis , the disease active index, colon mucosa damage index and score, intestine weight index, spleen index, thymus index, serum hemolysin level, interleukin - 17, interleukin -4 level in colon were determined, pathological changes in colon and spleen were observed by lightmicrescopy after HE staining. RESULTS： The colonicmucosa of UC mouses showed hyperemia, ulcers and infiltrated inflammatory cells, spleen white pulp atrophy. PPPE attenuated the severity of gross lesions, reduce DAI, CMDI. IWI value and IL - 17 concentration in colon, increase serum hemolysin level significantly. CONCLUSIONS ： PPPE exerts a protective effect on colon injury in TNBS - induced UC mouses, which may be due to increasing humoral immunity and decreaseing inflammatory cytokines IL - 17.