激肽释放酶制取纯化工艺的改进试验

An Experiment of the Improved Purificatory Technology of Extracting Kininase

为探寻控制胰酶活化的最佳条件 ,提高激肽释放酶精品的比活力 ,进行了激肽释放酶制取纯化工艺的改进试验 .首先绞碎胰腺 ,经提取、活化后用丙酮沉淀、脱水、干燥得胰酶粗品 .粗品经溶解、提取、上柱层析、透析脱盐、冻干即得激肽释放酶精品 .与传统工艺相比 ,新工艺粗品收率提高了 3% ,精品比活力提高了 10倍 。

This experiment aimed at finding out the best condition to control pancreatin activation and raise the specific activity of fine Kininase.Firstiy,the pancreas was cut up, abstracted 、activated, precipited with Acetone,dewatered and dried.Then the Primary engyme was dissolved and abstracted.After chomatography, dialysis for desalting and lyophiligation,fine Kininase was obtained. Compared with traditional technology, the new technology raised the recovery rate of primary engyme by 3% and increased engyme specific activity by 10 times. This met the standard of clinical practice.