血管低温保存的初步研究

PLIOT STUDY ON THE CRYOPRESERVATION OF ARTERY

血管组织的低温保存具有重要的药疗和研究价值。低温保存过程中是离不开抗冻剂的。合理地选择和配制抗冻剂对低温保存的成败是至关重要的。本文主要研究了抗冻剂 Ｄ Ｍ Ｓ Ｏ 浓度变化对小牛主动脉保存后细胞活性和功能的影响。实验结果表明： Ｄ Ｍ Ｓ Ｏ 浓度为１５％ 时， 血管保存效果最好， 血管组织的超微结构有７０％保存完好， 血管对１０－ ６ Ｍｎｏｒｅｐｉｎｅｐｈｒｉｎｅ 的收缩力达到新鲜血管的８０％ 左右， 对ａｃｅｔｙｌｃｈｏｌｉｎｅ 的舒张力达到３０—４０％ ， 但动脉血管的其他功能保持完好。

The cryopreservation of blood vessels is important both for surgical and for pharmacological applications.In the process of cryopreservation cryoprotectant should be used.The kind of cryopretectant and the method of introduction and removal of dimethyl sulfoxide are important for the survival of artery.In this paper we report the tolerance of cow artery to dimethyl sulfoxide in concentrations up to 30%.The maximum concentration achieved without damaged was 15%.The maximal recovery of contractile force in response to 10 -6 M norepinephrine was 30-40%;relaxation to acetylcholine was 80% of control,and an estimated 71% of endothelial cells survived with minimal ultrastructural change.