基于微带阵列电极的微型葡萄糖传感器研究

Study on the Microband Array Electrode_based Glucose Microsensor

用微电子薄膜技术制作了微带阵列电极（ＭＡＥ），考察了该电极在铁氰化钾、过氧化氢溶液中的电化学行为．在微带阵列电极表面，修饰一层全氟代磺酸酯（Ｎａｆｉｏｎ）膜作为基底电极，并把电子介体二茂铁及葡萄糖氧化酶固定在基底电极上制备了微型葡萄糖传感器．探讨了微酶电极对葡萄糖氧化过程的催化作用．该微酶电极响应时间小于１０ｓ，检测线性上限为８ｍｍｏｌ／Ｌ．

The microband array electrode(MAE) consisting of 11 Pt microband of 5 m in wide, 2000 m long and 85 m gap was fabricated with a thin film techology .The MAE was characterized by means of cyclic voltammetry in a solution of K3Fe(CN)6 and H2O2. The results showed higher current density on MAE than on conventional Pt eletrode due to the strong edge effect of the former. A glucose microsensor was constructed with the substrate electrode of microband array. Microband array electrode was modified by Nafion followed by the immobilization of ferrocene onto Nafion layer as the electron transfer mediator,finally a glucose microsensor was constructed by using bovine serum albumin and glutaraldehyde to immobilize glucose oxidase onto the surface of Nafion_Fc modified microband array electrode. The upper detection limit of the microsensor was 8.0 mmol/L and the response time was less than 10s.No significant variation was observed within two weeks use in detection.