中药对围绝经期大鼠脑组织中NOS mRNA的调节作用

Regulatory effect of traditional Chinese medicine on nitric oxide synthase mRNA in brain tissues of perimenopausal rats

目的:探讨中药更年乐佳液对围绝经期大鼠脑组织中一氧化氮合酶(Nitric oxide synthase,NOS)mRNA表达的影响。方法:将50只12～15月龄SD雌鼠随机分为5组:更年乐佳液高剂量组(乐高组)、更年乐佳液中剂量组(乐中组)、更年乐佳液低剂量组(乐低组)、空白对照组、尼尔雌醇组,每组10只。并以10只雌性成年未孕4～5月龄大鼠作为正常对照组。将麻醉好的动物取下丘脑组织,采用逆转录聚合酶链反应(RT-PCR)测定NOS mRNA的表达情况。结果:乐高组NOSmRNA表达总阳性率为80%;乐中组总阳性率为70%;尼尔雌醇组总阳性率为80%;正常对照组总阳性率为80%;均明显高于空白对照组50%(P<0.05)。而乐低组总阳性率仅为60%,与空白对照组相比无统计学差异(P>0.05)。结论:更年乐佳液可以促进神经元型NOS mRNA的表达。

Objective：To explore the effect of Gengnianlejia on expression of nitric oxide synthase（NOS） mRNA in brain tissues of perimenopausal rats. Methods：50 female SD rats aged 12～15 months old were divided into five groups randomly：high dose group of Gengnianlejia,middle dose group of Gengnianlejia,low dose group of Gengnianlejia,blank control group and nilestriol group,10 rats in each group;10 adult female rats aged 4～5 months old who were unpregnancy were regarded as normal control group;the hypothalamus tissues of the rats were obtained,then the expression of NOS mRNA was detected by RT-PCR. Results：The total positive rates of NOS mRNA expression in high dose group of Gengnianlejia,middle dose group of Gengnianlejia,nilestriol group and normal control group were 80%,70%,80% and 80%,respectively,which were significantly higher than that in blank control group（50%）（P〈0.05）;the total positive rate of NOS mRNA expression in low dose group of Gengnianlejia was 60%,compared with blank control group,there was no significant difference（P〉0.05）. Conclusion：Gengnianlejia can promote the expression of neurogenic NOS mRNA.