HBV宫内感染胎盘组织TLR-4、HBD-2及孕妇外周血IFN-γ的相关性

Correlation between TLR-4,HBD-2 in placental tissues and IFN-γ in peripheral blood of pregnant women with HBV intrauterine infection

目的:研究乙型肝炎病毒宫内感染TLR-4、HBD-2及IFN-γ水平及相关性。方法:选择乙肝标志物HB-sAg、HBeAg为阳性且肝功能正常的孕妇57例为试验组,根据脐血清乙肝两对半结果,分为宫内感染组26例,宫内未感染组31例,外周血血清HBV标志物均为阴性的正常分娩孕妇20例为对照组,采用ELISA法检测孕妇外周血血清IFN-γ水平;SYBRGreenⅠ实时荧光定量RT-PCR的方法检测TLR-4,HBD-2在孕妇胎盘组织中的表达,选择GAPDH为内参。结果:①宫内感染组TLR-4,HBD-2,IFN-γ的表达均显著低于HBV宫内未感染组及对照组(P<0.01)。宫内未感染组和对照组之间的差异无统计学意义(P>0.05)。②胎盘TLR-4、HBD-2与孕妇外周血IFN-γ水平均成显著正相关关系(r=0.815,P<0.01;r=0.685,P<0.05)。结论:TLR-4、HBD-2下调与IFN-γ水平下降相关,导致HBV宫内感染。

Objective：To study the correlation between TLR-4,HBD-2 and IFN-γ in pregnant women with HBV intrauterine infection. Methods：57 pregnant women with positive HBsAg and HBeAg and normal hepatic function were selected as experimental group,then they were divided into intrauterine infection group（26 cases） and non-intrauterine infection group（31 cases） according to the results of two half-and-half examination of umbilical serum,20 normal pregnant women with negative HBV markers in peripheral serum were selected as control group;ELISA was used to detect the level of IFN-γ in the serum of peripheral blood;SYBR Green Ⅰ real-time fluorescent quantitative PCR was used to detect the expression levels of TLR-4 and HBD-2 in placental tissues of pregnant women. Results：The expression levels of TLR-4,HBD-2 and IFN-γ in intrauterine infection group were significantly lower than those in non-intrauterine infection group and control group（P〈0.01）,but there was no significant difference between non-intrauterine infection group and control group（P〉0.05）;there was a significant positive correlation between TLR-4,HBD-2 levels in placental tissues and IFN-γ level in the serum of peripheral blood（γ=0.815,P〈0.01;γ=0.685,P〈0.05）. Conclusion：The down-regulation of TLR-4 and HBD-2 is related to the decrease of IFN-γ level,which induce HBV intrauterine infection.