痕量元素与DNA体内和体外结合的多种示踪法研究

MULTITRACER STUDY ON IN VIVO AND IN VITRO BINDING OF TRACE ELEMENTS WITH MOUSE LIVER DNA

采用多种示踪法研究了 Ｂｅ、 Ｓｃ、 Ｍ ｎ、 Ｃｏ、 Ｚｎ、 Ａｓ、 Ｓｅ、 Ｒｂ、 Ｓｒ、 Ｙ、 Ｚｒ、 Ｔｃ、 Ｒｕ 和 Ｒｈ 等 １４ 种痕量元素与鼠肝 Ｄ Ｎ Ａ 的体内、组织培养和体外结合。除了 Ａｓ、 Ｒｕ 和 Ｒｈ 以外，观察到大多数元素在体内都能与鼠肝 Ｄ Ａ Ｎ 结合；在组织培养实验中，只有 Ａｓ 和 Ｒｂ 不与 Ｄ Ｎ Ａ 结合；在体外实验中，全部元素都与 Ｄ Ｎ Ａ 结合，其中 Ｒｂ、 Ｓｅ、 Ｚｒ、 Ｒｕ、 Ａｓ 最弱。总体上，体外的结合最强，组织培养其次，体内最弱。体内与组织培养的结合情况比较类同。

In vivo,semi in vivo and in vitro binding of a series of trace elements (Be,Sc,Mn,Co,Zn,As,Se,Rb,Sr,Y,Zr,Tc,Ru and Rh)is studied by the multitracer technique.The corresponding nuclides in the multitracer solution used are 7 Be, 46 Sc, 54 Mn, 58 Co, 65 Zn, 74 As, 75 Se, 83 Rb, 85 Sr, 88 Y, 88 Zr, 95 Tc m, 103 Ru and 102 Rh m.It is found that most elements bound mouse liver DNA in vivo except As, Ru and Rh.In the semi in vivo experiment,only elements Rh and As are not observed to be bound with DNA.In the in vitro experiment,DNA bound with all elements,among which Rb,Se,Zr,Ru and As showed very slight binding.In comparison,the binding in vitro is the strongest,semi in vevo the medium and in vivo the weakest.