聚四氨基酞菁铜微型传感器及其在一氧化氮测定中的应用

Electrochemical Microsensor Based on Electropolymerized Film of Tetraaminophthalocyanine Copper and Its Application in Nitric Oxide Determination

利用电化学聚合的方法制备了聚四氨基酞菁酮[Cu(TAPc)]微型传感器,并探讨了微型传感器对一氧化氮(NO)的电化学响应.结果表明,电化学聚合Cu(TAPc)微型传感器对NO具有良好的催化氧化作用.示差脉冲伏安法(DPV)和示差脉冲安培法(DPA)的结果表明,该传感器对NO的响应具有较低的检测限和较高的灵敏度.NO的浓度在1.20×10^(-8)～1.00×10^(-6)mol/L范围内与传感器的电流响应呈线性关系,相关系数为0.9998;检出限为6.00×10^(-9)mol/L.生物体内常见共存物质如抗坏血酸、儿茶酚胺类神经递质及其代谢产物、NO的降解产物NO_2^-等不干扰NO的测定.所研制的微型传感器可以满足NO的活体分析的要求.

The preparation of electrochemical microsensor based on an electropolymerized film of tetraaminophthalocyanine copper and its response to NO were described in this work. We have found that the microsensor based on the electropolymerized film of tetraaminophthalocyanine copper Cu(TAPc) and Nation shows a high activity for the oxidation of NO with a high sensitivity and selectivity for NOdetermination. NO concentration is linear with the oxidation current in the range of 1.20×10-8 to 1.00×10-6 mol/L with a calcuated detection limit of 6.00×10-9 mol/L. The microsensor could be free fromthe interference from some biological substances coexisting in biological fluid, such as ascorbate, uric acid, nitrite and matebolites of monoamines. The microsensor could meet the requirements of in vivo measurements of NO.