期刊文献+

帕瑞昔布治疗大鼠局灶性脑缺血-再灌注损伤最佳剂量及有效时间窗 被引量:3

The optimal therapeutic dose and time window of parecoxib against focal cerebral ischemia-reperfusion injury in rats
下载PDF
导出
摘要 目的确定帕瑞昔布治疗局灶性脑缺血-再灌注损伤最佳剂量及有效时间窗。方法最佳剂量部分:60只雄性SD大鼠随机均分为缺血-再灌注组(I1组)、溶剂组(V组)及P1、P2、P3、P4组;治疗时间窗部分:50只雄性SD大鼠随机均分为I2、T1、T2、T3及T4组。I1与I2组为线栓法局灶性脑缺血-再灌注模型组,V组为生理盐水组,P1、P2、P3与P4组为帕瑞昔布5、10、20及40mg/kg,均于置入线栓前15min腹腔给药;T1、T2、T3、T4组分别于置入线栓前15min、后1.5、3、6h腹腔注射帕瑞昔布20mg/kg。缺血-再灌注后24h行神经行为学(NBS)评分及脑切片TTC染色计算脑梗死容积百分比。结果 P2和P3组NBS评分明显高于I1组(P<0.05);P3组脑梗死容积百分比最低(P<0.05)。T1、T2、T3组NBS评分明显高于I2组,T1、T2、T3组脑梗死容积明显低于I2组(P<0.05或P<0.01)。结论帕瑞昔布对大鼠脑缺血-再灌注损伤有治疗作用,其最佳剂量为20mg/kg,有效时间窗为再灌注后3h。 Objective To determine the optimal therapeutic dose and time window of Parecoxib on focal cerebral ischemia reperfusion injury in rats. Methods Part I, sixty male SD rats were randomly divided into six groups: groups I 1 , V , P1 , P2, P3 and P4 Parecoxib or vehicle was intraperitoneally administered at 15 rain before ischemia reperfusion injury by middle cerebral artery occlusion. Part Ⅱ , Fifty male SD rats were randomly divided into five groups: groups I2 ,T1 ,T2 ,T3, T1. Parecoxib were treated randomly at 15 rain(group T1 ) before or 1.5 h(group T2 ), 3 h(group T3 ) and 6 h (group T4 ) after injury. The neurologic behavior score (NBS) was evaluated at 24 h after reperfusion and the cerebra] infarct volume percentage (CIVP) was assessed by TTC staining. Results The NBS in the groups P2 and P3 was significantly higher than that in group I1 (P〈0.05) ; the CIVP in the group P3 was the lowest (P〈0.05). Compared with I2, the behavioral and histological outcomes were improved at groups T1, T2 and T3 (P〈0.05 or P〈0.01). Conclusion Parecoxib processes therapeutic effect on ischemia reperfusion injured brain in rats, the optimal dose is 20 mg/kg, and the effective time window is 3 h after ischernia.
出处 《临床麻醉学杂志》 CAS CSCD 北大核心 2011年第3期281-283,共3页 Journal of Clinical Anesthesiology
关键词 COX-2抑制药 帕瑞昔布 缺血再灌注 剂量效应关系 时间窗 COX-2 inhibitor Parecoxib Ischemia-reperfusion Doseresponse relationship Time window
  • 引文网络
  • 相关文献

参考文献8

  • 1Iadecola C,Niwa K,Nogawa S,et al.Reduced susceptibility to ischemic brain injury and N-methyl-D-aspartate-mediated neurotoxicity in cyclooxygenase-2-deficient mice.Proc Natl Acad Sci USA,2001,98:1294-1299.
  • 2Strauss KI,Marini AM.Cyclooxygenase-2 inhibition protects cultured cerebellar granule neurons from glutamate-mediated cell death.J Neurotrauma,2002,19:627-638.
  • 3李扬,杨博,陈绍洋,汪晨,路志宏,马福成,熊利泽.参附注射液预处理对大鼠脑缺血-再灌注损伤后HSP70和HSP90表达的影响[J].临床麻醉学杂志,2010,26(1):51-53. 被引量:9
  • 4Dembo G,Park SB,Kharasch ED.Central nervous system concentrations of cyclooxygenase-2 inhibitors in humans.Anesthesiology,2005,102:409-415.
  • 5Abroad M,Zhang Y,Liu H,et al.SProlonged opportunity for neuroprotection in experimental stroke with selective blockade of cyclooxygenase-2 activity.Natl Aead Sci,2009,1279:168-173.
  • 6Abroad M,Saleem S,Zhuang H,et al.1-hydroxy PGE reduces infarction volume in mouse transient cerebral ischemia.Eur J Neurosci,2006,23:35-42.
  • 7Sugimoto Y,Narumiya S.Prostaglandin E receptors.J Biol Chem,2007,282:11613-11617.
  • 8Lipton P.Ischemic cell death in brain neurons.Physiol Rev,1999,79:1431-1568.

二级参考文献6

共引文献8

同被引文献22

  • 1高枫,肖冰.帕瑞昔布对输尿管梗阻后肾功能的保护作用[J].陕西医学杂志,2008,37(11):1488-1489. 被引量:7
  • 2Wang JQ,Kon J,Mogi C. TDAG8 is a proton-sensing and psychosine-sensitive G-protein-coupled receptor[J].Journal of Biological Chemistry,2004,(44):45626-45633.
  • 3Longa EZ,Weinstein PR,Carlson S. Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,(01):84-91.doi:10.1161/01.STR.20.1.84.
  • 4Mutze K,Langer R,Becket K. Histone deacetylase(HDAC)1 and 2 expression and chemotherapy in gastric cancer[J].Annals of Surgical Oncology,2010,(12):3336-3343.
  • 5Derouesne C,Cambon H,Yelnik A. Infarcts in the middle cerebral artery territory.Pathological study of the mechanisms of death[J].Acta Neurologica Scandinavica,1993,(05):361-366.
  • 6Choi JW,Lee SY,Choi Y. Identification of a putative G protein-coupled receptor induced during activation-induced apoptosis of T cells[J].Cellular Immunology,1996,(01):78-84.
  • 7Im DS,Heise CE,Nguyen T. Identification of a molecular target of psychosine and its role in globoid cell formation[J].J Ceil Biol,2001,(02):429-434.
  • 8McGuire J,Herman JP,Ghosal S. Acid-sensing by the T cell death-associated gene 8(TDAG8)receptor cloned from rat brain[J].Biochemical and Biophysical Research Communications,2009,(03):420-425.doi:10.1016/j.bbrc.2009.05.133.
  • 9叶伟娣,周海燕.术前肌内注射帕瑞昔布钠对视网膜脱离术术后疼痛及围术期血液皮质醇、前列腺素E2、白细胞介素-6的影响[J].上海医学,2010,33(2):169-171. 被引量:8
  • 10王庆,李真,王志鹏,崔灿.帕瑞昔布钠在腹腔镜结肠癌根治术中的超前镇痛作用[J].南方医科大学学报,2010,30(11):2556-2557. 被引量:35

引证文献3

二级引证文献5

;
使用帮助 返回顶部