摘要
目的确定帕瑞昔布治疗局灶性脑缺血-再灌注损伤最佳剂量及有效时间窗。方法最佳剂量部分:60只雄性SD大鼠随机均分为缺血-再灌注组(I1组)、溶剂组(V组)及P1、P2、P3、P4组;治疗时间窗部分:50只雄性SD大鼠随机均分为I2、T1、T2、T3及T4组。I1与I2组为线栓法局灶性脑缺血-再灌注模型组,V组为生理盐水组,P1、P2、P3与P4组为帕瑞昔布5、10、20及40mg/kg,均于置入线栓前15min腹腔给药;T1、T2、T3、T4组分别于置入线栓前15min、后1.5、3、6h腹腔注射帕瑞昔布20mg/kg。缺血-再灌注后24h行神经行为学(NBS)评分及脑切片TTC染色计算脑梗死容积百分比。结果 P2和P3组NBS评分明显高于I1组(P<0.05);P3组脑梗死容积百分比最低(P<0.05)。T1、T2、T3组NBS评分明显高于I2组,T1、T2、T3组脑梗死容积明显低于I2组(P<0.05或P<0.01)。结论帕瑞昔布对大鼠脑缺血-再灌注损伤有治疗作用,其最佳剂量为20mg/kg,有效时间窗为再灌注后3h。
Objective To determine the optimal therapeutic dose and time window of Parecoxib on focal cerebral ischemia reperfusion injury in rats. Methods Part I, sixty male SD rats were randomly divided into six groups: groups I 1 , V , P1 , P2, P3 and P4 Parecoxib or vehicle was intraperitoneally administered at 15 rain before ischemia reperfusion injury by middle cerebral artery occlusion. Part Ⅱ , Fifty male SD rats were randomly divided into five groups: groups I2 ,T1 ,T2 ,T3, T1. Parecoxib were treated randomly at 15 rain(group T1 ) before or 1.5 h(group T2 ), 3 h(group T3 ) and 6 h (group T4 ) after injury. The neurologic behavior score (NBS) was evaluated at 24 h after reperfusion and the cerebra] infarct volume percentage (CIVP) was assessed by TTC staining. Results The NBS in the groups P2 and P3 was significantly higher than that in group I1 (P〈0.05) ; the CIVP in the group P3 was the lowest (P〈0.05). Compared with I2, the behavioral and histological outcomes were improved at groups T1, T2 and T3 (P〈0.05 or P〈0.01). Conclusion Parecoxib processes therapeutic effect on ischemia reperfusion injured brain in rats, the optimal dose is 20 mg/kg, and the effective time window is 3 h after ischernia.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2011年第3期281-283,共3页
Journal of Clinical Anesthesiology