毛细管区带电泳激光诱导荧光间接检测环腺苷单磷酸和环鸟苷单磷酸

Separation of Cyclic-adenosine monophosphate(cAMP) and Cyclic-guanosine monophosphate(cGMP) by Capillary Zone Electrophoresis With Indirect Laser-induced Fluorescence Detection

研究了影响毛细管区带电泳激光诱导荧光间接检测环腺苷单磷酸和环鸟苷单磷酸的实验条件.cAMP和cGMP的线性范围分别为30～500mg/L和15～500mg/L,其最低检测限分别为9.0mg/L和0.5mg/L;当浓度为125mg/L时,cAMP和cGMP峰面积的RSD为4.45%和6.21%(n=4);其迁移时间的日间RSD分别为2.92%和2.04%(n=5)

This paper reports the optimum conditions of separation and determination of cyclic-adenosine monophosphate( cAMP ) and cyclic-guanosine monophosphate(cGMP) by capillary zone electrophoresis with indirect laser-induced fluorescence detection. The linear ranges of determination for cAMP and cGMP are 30-500 mg/L and 15-500 mg/L. The concentration limit of detection ( CLOD) for cAMP and cGMP are 9.0 mg/L and 0.5 mg/L. The RSD of peak areas of cAMP and cGMP are 4.5% and 6.2% ( n - 4) in the 125 mg/L; the intra-day RSD of the retention times of cAMP and cGMP are 2.9% and 2. 0% ( n = 5) , respectively.