摘要
丝状微生物体形态对工业过程(如甾体激素、抗生素的发酵生产)的影响,已日益引人注目.利用丝状霉菌(如 Metarhizium sp.)产生的11α-羟化酶催化甾体底物16α,17α-环氧黄体酮(16α,17α-epoxy-4-pregnene-3,20-dione)羟化转化的反应,是甾体激素类药物合成中最重要的微生物反应之一.霉菌菌体形态包括粗细、长势(丰满或萎缩)、分枝程度和表面状况等.环境参数(如剪切力、氧分压等)影响菌体形态的形成;形成的形态反过来又会强烈影响环境的性质(如培养基的流变性、菌丝球内部的理化性质等),进而影响菌体的生长与性能.其中性能评价及环境监测(如生物传感)手段已日趋成熟,而定量解析形态则较困难.用计算机视觉替代人的视觉,提供了一条客观、准确、迅速的解析途径,值得探索.
Computer aid in the study of biotransformation process is a new approach to comparatively comprehensive understanding of the course of mycelia life. On the basis of fractal and spatial gray level co - occurrence matrix models, is possible to describe the mycelial morphology quantitatively by taking advantage of Computer Image Texture Analysis Technique. Plotting the calculated fractal dimension and texture entropy features from the morphological images obtained at different phases of growth, gives a clear correlation, that is the dependency of the mycelial morphology on the cultivation conditions and stages.
出处
《化工学报》
EI
CAS
CSCD
北大核心
1999年第4期573-576,共4页
CIESC Journal
基金
国家自然科学青年基金(NO.29606008)
天津市自然科学基金(No.993607111)资助项目
