非小细胞肺癌组织中ZO-1的表达及其临床意义

Expression and Clinical Significance of ZO-1 in Patients with Non-small Cell Lung Cancer

背景与目的已有的研究表明紧密连接蛋白-1(zonula occluden-1,ZO-1)表达和肿瘤细胞的生长和转移存在密切联系。本研究旨在探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中ZO-1表达的临床意义。方法应用实时荧光定量PCR、Western blot和免疫组化检测101例NSCLC癌组织及癌旁组织中ZO-1mRNA和蛋白表达,以61例肺部良性病变患者作为对照。结果 ZO-1mRNA在癌组织和肺良性病变组织间差异具有统计学意义(P<0.01)。Western blot显示:ZO-1蛋白在癌组织、癌旁组织、肺良性病变组织的相对表达量之间均存在明显差异(P<0.01或P<0.05)。免疫组化显示:ZO-1平均光密度在癌组织、癌旁组织和肺良性病变组织中分别为69.55±17.13、246.39±83.15、330.93±72.44,组间差异均具有统计学意义(P<0.01)。伴有淋巴结转移与不伴淋巴结转移肺癌组织ZO-1mRNA表达水平比较有显著性差异(t=-5.07,P<0.01),肺癌患者术后2年内生存组和2年内死亡组间ZO-1mRNA表达水平比较差异具有统计学意义(t=5.61,P<0.01)。结论 ZO-1mRNA和蛋白在NSCLC癌组织中表达降低,并与淋巴结转移的术后生存有密切关系。

Background and objective It has been proven that the expression of ZO-1 （zonula occluden-1） was correlated with the growth and metastasis of cancer. The aim of this study is to investigate the relationship between ZO-1 gene expression and clinicopathologic parameters and the survival rates in the patients with non-small cell lung cancer （NSCLC）. Methods The expression levels of ZO-1 gene in 101 patients with NSCLC and lung tissues of 61 patients with benign lung disease were detected by real-time PCR, Western blot and immunohistochemical techniques. Results There was a significant statistic difference of ZO-1 mRNA expression levels between carcinoma group and control group （P〈0.01）. In carcinoma group, adjacent group and control group, ZO-1 protein expression levels were with significantly statistic difference between each other （P〈0.01 or P〈0.05）. There was a significantly statistic difference between ZO-1 mRNA expression levels in survival during 2 years follow-up group and death during 2 years follow-up group （t=-5.61, P〈0.01）. Conclusion ZO-1 is a valuable predictive marker for NSCLC patients.