毛细管电泳环糊精添加剂立体选择性差异的研究

A Study of Recognition Mechanism of Enantiomeric Separation of Tulobuterol in Cyclodextrinmediated Capillary Zone Electrophoresis

对在毛细管区带电泳环糊精手性拆分体系中手性药物妥布特罗的分离机理进行了探讨。分别测定了α、β、γ环糊精和二甲基、三甲基、羟丙基β环糊精与药物对映体形成包结配合物的稳定常数以及手性拆分过程中的热力学函数的变化。

The mechanism of enantioselectivity in cyclodextrinmediated capillary zone electrophoresis (CDCZE) on basic drug tulobuterol was discussed. The equilibrium constants of hostguest complexion of tulobuterol enantiomers with cyclodextrin, cyclodextrin, cyclodextrin, 2,6dimethylcyclodextrin, 2,3,6trimethylcyclodextrin and 2hydroxypropylcyclodextrin, and the thermodynamic parameters for chiral recognition procedure were determined respectively. Selectivity calculated from the ratio of binding constants of two enantiomers is found to be in the same order of maximum electrophortic mobility difference between the enantiomers responding with cyclodextrin additive. The experimental value of optimal concentration of cyclodextrin agrees with the one calculated from Author the equation C\-opt=1/ (K\-1K\-2)\+1/2.