睑板腺癌裸鼠移植瘤模型的建立及其生物学特性的研究

Preliminary study on the establishment of the animal model and biological activity of human being mebomian gland carcinoma

目的探讨建立睑板腺癌裸鼠移植瘤模型的方法，研究肿瘤生物学特性。方法采用组织块法，将4例新鲜人睑板腺癌组织块直接接种于8只裸鼠皮下。观察成瘤时间、肿瘤生长情况。待移植瘤体成活并生长至一定大小后，取部分瘤体进行HE染色，苏丹Ⅲ染色以及广谱细胞角蛋白、上皮膜抗原免疫组织化学染色。结果新鲜人睑板腺癌组织能在裸鼠皮下存活并生长，原代移植瘤第5周开始生长，第10周加速生长。HE染色光镜观察，肿瘤呈片状或团状排列，多数细胞呈睑板腺细胞样分化，瘤细胞比较大，呈多边形，胞质呈泡沫状，细胞核位于中央稍向细胞周边偏位，核异型性明显，核分裂象易见。苏丹Ⅲ染色，细胞质和细胞间隙内可见橙红色脂肪颗粒。免疫组织化学染色，广谱细胞角蛋白、上皮膜抗原均为阳性表达。结论采用组织块法成功建立了人睑板腺癌的裸鼠皮下移植瘤模型。该移植瘤良好地保留了人睑板腺癌的生物学特性。

Objective To explore the methods to establish a xenografted model of mebomian gland carcinoma in nude mice, then find the best method to establish the animal model. The last purpose of this study was to establish a suitable animal model for clinic therapy and basic research of mebomian gland carcinoma. Methods Small pieces of mebomian gland carcinoma tissue from four patients were transplanted subcutaneously in the flanks of eight nude mice through a skin incision. Nature of transplantation tumor including formation, growth and size were observed. The transplanted tumors were stained with hematoxylineosin,Sudan Ⅲ and hnmunohistochemistry. Results The human mebomian gland carcinoma tissue can survived in nude mice. The pathologic examination revealed that the transplanted tumor was arranged in mass. The tumor cells were round or multisided. The proportion of the nuclei and cytoplasm was imbalance. Nuclear division could be easily observed. The test of Sudan Ⅲ was positive. Immunohistochemical observation： the results showed that both CK and EMA were positive. Conclusions The subcutaneously xenotransplanted tumor model of human mebomian gland carcinoma with tissue explant in nude mice was successfully established. The transplanted tumor maintained the properties of human mebomian gland carcinoma.