单核细胞趋化蛋白1和骨形成蛋白7在病理性瘢痕中的表达

Expression of monocyte ehemoattraetant protein-1 and bone morphogenic protein-7 in pathologic scars

背景:单核细胞趋化蛋白1是新近明确的对单核/巨噬细胞有趋化和激活双重作用的趋化因子,骨形成蛋白7作为一种新发现的纤维化负性调节因子逐渐成为抗组织纤维化治疗的研究热点,但两者对病理性瘢痕形成中组织纤维化作用的研究至今鲜有报道。目的:研究单核细胞趋化蛋白1,骨形成蛋白7在病理性瘢痕中的表达水平。方法:采用免疫组织化学方法检测单核细胞趋化蛋白1、骨形成蛋白7在25例瘢痕疙瘩、30例增生性瘢痕、24例非病理瘢痕和20例正常皮肤组织中的表达水平。所有标本均来自2008-07/2010-01郑州大学第一附属医院整形外科住院患者,且均无皮肤疾病、结缔组织病、传染病、恶性肿瘤和其他重要脏器疾病,术前无射线治疗、激光治疗及免疫治疗史,其中所取瘢痕组织来自于临床诊断明确的瘢痕患者。结果与结论:单核细胞趋化蛋白1在瘢痕疙瘩、增生性瘢痕中的阳性表达率均高于非病理性瘢痕与正常皮肤组织(P<0.05),骨形成蛋白7阳性表达率均降低(P<0.05),两者阳性表达率在病理性瘢痕(瘢痕疙瘩和增生性瘢痕)中呈明显负相关(r=-0.639,P<0.01)。结果显示,在病理性瘢痕的形成过程中单核细胞趋化蛋白1表达上调,而骨形成蛋白7表达下调。

BACKGROUND：Monocyte ehemoattraetant protein-1 （MCP-1） has been shown chemotaxis and activation effect on mononuclear/macrophage.As a newly found negative-regulatory factor,bone morphogenic protein-7 （BMP-7） has aroused increasing attention in the treatment of tissue fibrosis.However,the effects of MCP-1 and BMP-7 on tissue fibrosis during pathologic scars remain poorly understood.OBJECTIVE：To investigate the expression of MCP-1 and BMP-7 in pathologic scars.METHODS：SP immunohistochemical method was used to detect the expressions of MCP-1 and BMP-7 in 25 cases of keloid,30 cases of hypertrophic scars,24 cases of non-pathologic scar and 20 cases of normal skin tissues.All specimens obtained from plastic surgery patients that had no skin disease,connective disease,infectious disease,malignant tumor and other important viscera diseases,no radiation therapy,laser therapy and immunotherapy before surgery in the First Affiliated Hospital of Zhengzhou University.Meanwhile,the scar tissues were taken from the patients who were diagnosed clearly by the clinic,and they were confirmed by pathology.RESULTS AND CONCLUSION：The positive rates of MCP-1 in keloid group was higher than that of the hypertrophic scar and normal skin groups （P0.05）,but the positive rates of BMP-7 was decreased （P0.05）.There was a negative correlation between MCP-1 and BMP-7 expression in pathologic scars （keloid and hypertrophic scars） （r=-0.639,P0.01）.Results demonstrated that MCP-1 expression up regulated and BMP-7 expression down regulated during development of pathologic scars.