血小板衍化生长因子β对体外人牙周膜细胞增殖分化的影响

Effect of platelet-derived growth factor beta on in vitro proliferation and differentiation of human periodontal ligament cells

背景:牙周支持组织破坏及附着丧失是导致失牙的最主要原因,细胞因子能促进牙周组织再生形成新附着。目的:观察血小板衍化生长因子β在人牙周膜成纤维细胞增殖过程中的作用。方法:体外利用组织块法培养人牙周膜成纤维细胞,对传代后细胞进行组织来源鉴定,通过波丝蛋白、角蛋白免疫组化染色进行定性分析,并加入生物因子血小板衍化生长因子β对人牙周膜成纤维细胞进行增殖诱导,采用MTT法测定吸光度值。结果与结论:加入质量浓度为10μg/L的血小板衍化生长因子β后,细胞增殖加速,在加入生长因子血小板衍化生长因子β后,实验组的吸光度显著高于对照组,说明血小板衍化生长因子β对人牙周膜成纤维细胞的增殖具有明显的促进作用。

BACKGROUND：Periodontal supporting tissues destroy and attachment losses are the main reasons for tooth loss.Cytokine can promote periodontal tissue regeneration.OBJECTIVE：To research the effect of platelet-derived growth factor-β （PDGF-β） in the human periodontal ligament cells proliferation.METHODS：Human fibroblasts were cultured with tissue block method in vitro.The passaged cells were identified by Bosi protein,keratin immunohistochemistry for qualitative analysis.PDGF-β was added to induce periodontal ligament cells proliferation,and the absorbance value was measured by MTT method.RESULTS AND CONCLUSION：The cell proliferation was accelerated after adding 10 μg/L PDGF-β factor,the absorbance value of the experimental group was greater than that of the control group （P0.05）.The findings demonstrated that PDGF-β can promote the proliferation of human periodontal ligament cells.