体外诱导间充质干细胞向心肌样细胞分化的研究:药物、微环境及方法

Differentiation of mesenchymal stem cells into cardiomyocyte-like cells in vitro:Drug,microenvironment and method

背景:心肌细胞不能再生,损害的心肌细胞不能修复和分化,临床中药或西药治疗、介入治疗和手术治疗都不能代替坏死的心肌,所以如何修复坏死心肌细胞是心血管研究领域的重要课题。间充质干细胞体外诱导可向心肌样细胞分化使人们看到了修复和再生受损心肌细胞的希望。目的:综述近年来体外诱导间充质干细胞分化为心肌细胞研究进展,探索其未来发展方向。方法:应用计算机检索CNKI、Elsevier-Sdol数据库,检索2000-01/2010-08关于体外诱导间充质干细胞分化为心肌细胞方法,在标题中以"间充质干细胞;诱导;心肌样细胞"和"mesenchymal stem cells,cardiomyocyte-like cells,induce,the progress of study"为检索词进行检索,共收集185篇相关文献,中文103篇,英文82篇。排除发表时间较早、重复及类似研究,纳入32篇符合标准的文献。结果与结论:很多实验研究证实间充质干细胞在体外一定条件下可诱导分化为心肌细胞,其诱导方法主要包括诱导分化剂诱导法及模拟心肌微环境诱导法。诱导分化为心肌细胞的效率仍有待提高,间充质干细胞分化为心肌细胞过程中的最佳诱导药物以及最佳体外微环境也在不断探索中。

BACKGROUND：Cardiomyocyte cannot regenerate. Damaged cardiomyocytes cannot be repaired and differentiate. Clinical traditional Chinese medicine or western medicine,interventional therapy and surgery therapy cannot substitute necrotic myocardium. How to repair necrotic cardiomyocytes is an important topic in the cardiovascular research. Mesenchymal stem cells （MSCs） induced in vitro can differentiate into cardiomyocyte-like cells,which brings hope for the repair and regeneration of damaged cardiomyocytes. OBJECTIVE：To analyze the progress of inducing MSCs into cardiomyocyte-like cells in vitro to know where we should go in the future. METHODS：A computer-based online search of articles on the methods of inducing MSCs into cardiomyocytes in vitro was performed in CNKI and Elsevier-Sdol （2000-01/2010-08） with the key words of ＂mesenchymal stem cells,cardiomyocyte-like cells,induce,the progress of study＂. A total of 185 articles on inducing MSCs into cardiomyocyte-like cells in vitro were collected,including 103 Chinese and 82 English. Outdate,repetitive and similar studies were excluded,and 32 were included. RESULTS AND CONCLUSION：Numerous studies have verified that MSCs could differentiate into cardiomyocytes in vitro under a certain condition. The induction method mainly contained differentiation inducer method and simulated cardiac microenvironment method. The efficiency of differentiation into cardiomyocytes should be elevated. The optimal induction drug and optimal in vitro microenvironment during the differentiation of MSCs into cardiomyocytes should be explored.