嗅鞘细胞联合神经干细胞移植治疗小儿脑发育不全

Combined transplantation of neural stem cells and olfactory ensheathing cells for the treatment of cerebral hypoplasia

背景:近年来,干细胞替代治疗为神经再生提供了新的方法,但干细胞移植治疗小儿脑发育不全的相关报道甚少。目的:验证嗅鞘细胞联合神经干细胞移植治疗小儿脑发育不全的有效性。方法:2009-10解放军第163医院收治了1例脑发育不全的患者,给予物理降温、神经营养、维持水电解质平衡、高压氧治疗的同时,以L3～4腰椎间隙为穿刺点,将配置好的神经干细胞约8×107个和嗅鞘细胞约4×107个缓慢注入蛛网膜下腔,每2周1次,2次为1个疗程,共治疗1个疗程。从体温恢复程度、双手精细动作、运动协调性、语言等方面来评价移植治疗效果。结果与结论:与移植前比较,嗅鞘细胞联合神经干细胞移植1个月后患儿体温恢复正常,四肢肌力明显下降,2个月后可以独自端坐,3个月后可以扶梯行走,有简单的语言表达。提示嗅鞘细胞联合神经干细胞移植治疗小儿脑发育不全是有效的,能恢复其中枢神经功能。

BACKGROUND：Recent years,stem cell replacement therapy furnishes a new method for neural regeneration. But until now,we have not found the reports about stem cell transplantation for treating cerebral hypoplasia. OBJECTIVE：To explore the effectiveness of combined transplantation of neural stem cells and olfactory ensheathing cells for the repair of cerebral hypoplasia. METHODS：In October,2009,a cerebral hypoplasia patient was enrolled at the 163 Hospital of the Chinese People＇s Liberation Army. We used the treatment of physical cooling,neurotrophic,maintenance of water-electrolyte balance,hyperbaric oxygen. We selected the point of puncture between the L3-4 of the spine and injected neural stem cells （about 8×107） and olfactory ensheathing cells （about 4×107） slowly into the subarachnoid space,once two weeks,twice as a course,totally one course. We assessed the results of transplantation treatment by drop of body temperature,fine motor,motor coordination and language expression. RESULTS AND CONCLUSION：The body temperature decreased to normal level,and muscular tension reduced obviously after one month,he could sit up by himself after two months,walk under the help of handrail,and have simple language expression after three months. These indicated that it is effective to treat cerebral hypoplasia and can recover central nerve function by combined transplantation of neural stem cells and olfactory ensheathing cells.