药用植物铃兰中吖丁啶-2-羧酸的测定

Determination of the Azetidine-2-Carboxylic Acid from Lily of the Valley

使用强阳离子交换柱分离、pH梯度洗脱、柱后衍生、荧光λex＝338nm和λem＝425nm检测的高效液相色谱法，成功地分析了药用植物铃兰中的吖丁啶－2－羧酸，方法回收率为96．4％。

This paper reports the separation and determination of the azetidine-2-carboxylic acid in lily of thevalley by high performance liquid chromatography (HPLC) with post--column derivatization and fluorescencedetection. The operating conditions were canon exchange resin column (30 cm × 0. 4 cm i. d. ) with buffer solution A [1. 96% (mass fraction) sodium citrate solution pH 3. 10±0. 02] and B [2. 1 % (mass fraction) sodium nitrate solution pH 9. 60± 0. 02] as mobile phase at flow-rate of 0. 4 mL/min, gradient elution, columntemperature 65℃,detection wavelength λex= 338 nm and λem= 425 nm. The retention time of azetidine-2-carboxylic acid was 5. 86 minutes. The recovery was 96. 4% and coefficient of variation was 0. 94%. Thismethod is simple,rapid and sensitive.