骨痹舒片对实验性膝骨关节炎病理形态及自由基的影响

Effect of Gubishu Tablet on Pathomorphology and Free Radical of Experimental Knee Osteoarthritis

目的:观察骨痹舒片对实验性膝骨关节炎病理形态的影响及对血清自由基的调节。方法:SD大鼠40只,采用改良Hulth法复制膝骨性关节炎模型,随机分为空白组、模型组、骨痹舒组、抗骨增生组,每组10只。每日灌胃1次,连续6周。6周后取股骨内髁关节软骨,经苏木-伊红、Masson、Safranin-O染色,光镜观察并行Mankin’s评分;测血清SOD、MDA、NO含量。结果:Mankin’s评分:模型组10.13±2.357,骨痹舒组6.75±1.753,组间比较差异有统计学意义(P<0.05);血清SOD、MDA、NO含量模型组分别为:21.315±8.633,12.619±1.51,70.125±3.786;骨痹舒组为:50.761±7.428,6.853±5.053,54.171±4.125,组间比较差异有统计学意义(P<0.05)。结论:骨痹舒片可通过提高SOD活性、有效清除氧自由基,抑制NO的过量释放及生物学效应,发挥减轻软骨破坏,延缓关节软骨退变的作用。

Objective： To observe the effect of Gubishu Tablet on pathomorphology and free radical of experimental knee osteoarthritis（OA）.Methods： Forty SD rats were randomly divided into 4 groups,blank group,model group,Gubishu group,and Kanggu Zengsheng group.Knee OA models were made by the modified Hulth method.Every group received a daily intragastric administration for 6 weeks,and then the articular cartilage of medial epicondyle femur was harvested to observe the pathological changes microscopically through the hematoxylin-eosin,Masson and Safranin-O-fast green-iron hematoxylin staining,and scoring based on the Mankin＇s.Meanwhile the level of the serum SOD,MDA,and NO was detected.Results： There was significant difference in Mankin＇s score and the level of the serum SOD,MDA,and NO between Gubishu group and model group（P 0.05）.Conclusion： Gubishu Tablet is effective in delaying degeneration of articular cartilage via inhibiting the oxygen free radical and NO and promoting the biological effect of SOD.