摘要
目的:通过牛膝中三萜皂苷类成分对破骨细胞分化的影响,探讨牛膝防治骨质疏松症的作用机理。方法:建立破骨细胞分化体外实验模型,在甲状旁腺素(PTH)的诱导下,骨髓细胞分化形成TRAP阳性破骨样细胞。在细胞培养过程中,分别加入2、20、200μM的各组三萜皂苷样品进行干预,考察每组样品对破骨细胞形成的影响。结果:考察的7个三萜皂苷类成分均能不同程度地抑制破骨细胞分化,其中竹节参苷IVa和木鳖子皂苷Ib抑制作用最强,且其抑制作用具有可逆性。结论:牛膝中三萜皂苷类成分可有效抑制破骨细胞形成,提示其可能作为骨吸收抑制剂用于防治骨质疏松症。
Objective： To observe the effect of triterpenoidal saponins from Achyranthes bidentata Bl.on osteoclast differentiation,and to elucidate their potential mechanism on antiosteoporosis. Methods： The bone marrow cells were cultured in the presence of PTH to form tartrate-resistant acid phosphatase（TRAP）-positive multinucleated cells,then triterpenoidal saponin samples at the concentration of 2,20,and 200 μM were added to test the effect on osteocalst differentiation. Results： Seven triterpenoidal saponins showed inhibitory activity on osteoclast differentiation,among which chikusetsusaponin Iva and momordin Ib showed more potent inhibitory activity,and the inhibitory activity were reversible.Conclusion： Triterpenoidal saponins from Achyranthes bidentata Bl.can inhibit osteoclast formation,indicating that they can be used as bone resorption inhibitor to treat osteoporosis.
出处
《中国中医骨伤科杂志》
CAS
2011年第3期9-10,13,共3页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
辽宁省教育厅科研项目(No.2008045)
教育部留学回国人员科研启动基金(No.2008-890)
关键词
牛膝
三萜皂苷
破骨细胞
骨质疏松
Achyranthes bidentata Bl.
Triterpenoidal saponins
Osteoclast
Osteoporosis
