溃疡性结肠炎患者血浆同型半胱氨酸和叶酸及维生素B12的检测水平及其临床意义

Significance of plasmic homocysteine, folate and Vitamin B12 in ulcerative colitis

目的探讨溃疡性结肠炎（UC）患者中血浆同型半胱氨酸（Hcy）、叶酸（FA）和维生素B12（VitB12）水平的改变及其临床意义。方法采用高效液相荧光检测法（HPLC—FD）检测112例UC患者和110名健康对照人群血浆Hcy水平．应用ELISA方法检测76例UC患者和12名健康对照人群血浆FA和VitB12水平。结果UC患者中血浆Hcy水平为（11．27±7．26）μmol／L，明显高于对照组的（8．19±4．81）μmol／L（P〈0．01）；UC患者中血浆Hcy水平与UC病情分度、病变范围及病程均无关（P〉0．05）。UC患者中血浆FA和VitB12水平分别为（7．64±1．95）nmol／L和（108．64±32．22）pmol／L，明显低于对照组[（9．14±1．23）nmol／L和（112．64±33．33）pmol／L，均P〈0．01]。高Hcy组患者血浆FA和VitB12水平显著低于低Hcy组（P〈0．05）。UC患者中血浆FA水平与UC病程呈负相关（P〈0．01）；而VitB12水平则与病程无相关性（P〉0．221）。结论UC患者血浆Hcy水平升高，可能与FA和VitB12水平下降有关。

Objective To investigate the clinical significance of plasmic homocysteine （Hcy）, folate （FA） and Vitamin B12 （VitB12） in patients with ulcerative colitis （UC）. Methods Plasmic Hcy in 112 cases of UC patients and 110 controls were detected by HPLC-FD method. Plasmic FA, VitB12 in 76 cases of UC patients and 12 controls were detected by enzyme-linked immunosorbent assay （ELISA） method. Results The level of plasmic Hey in UC patients was （11.27±7.26） μmol/L, significantly higher than that in controls [ （8.19±4.81） μmol/L, P〈0.05], and was not significantly correlated with disease index, extent and duration of UC （P〉0.05）. The level of FA and VitB12 in UC patients were （7.64±1.95） nmol/L and （108.64±32.22） pmol/L respectively, lower than those in controls [ （9.14± 1.23） nmol/L and （112.64±33.33） pmol/L,P〈0.05]. The level of plasmie Hcy was negatively correlated with the level of FA and VitB12 in UC patients （P〈0.05）. The level of plasmic FA decreased to some extent with UC disease duration. Conclusion Plasmie Hey is elevated in UC patients, which may be related to the decrease of FA and VitB12.