‘神马’菊花DREB1A基因的分离及同源遗传转化

Isolation and Homologous Genetic Transformation of DREB1A in Chrysanthemum cv.‘Jinba’

逆境诱导转录因子DREB1A基因在植物中的超量表达能够提高植株对低温、干旱、盐渍、高温等非生物胁迫的耐性。从菊花中克隆得到DgDREB1A基因,构建了转基因表达载体pBIG-DREB1A,并转入农杆菌LBA4404中;在建立了菊花高效再生体系的基础上,优化受体再生体系,通过根癌农杆菌介导将DgDREB1A基因转入切花菊‘神马’品种。PCR结果显示,在获得的38株卡那霉素抗性植株中有8株为阳性,表明外源基因已整合到转化植株基因组中,转化效率为3‰。本研究为培育综合抗逆性良好的菊花新品种奠定工作基础。

Overexpression of the stress-inducible transcription factor gene DREB1A in transgenic plants conferred strong tolerance to abiotic stresses,such as salt,low temperature,dehydration and heat stresses.We cloned DgDREB1A gene from chrysanthemum（Chrysanthemum morifolium Ramat.）cv.‘Jinba’,built transgene expression vector pBIG-DREB1A and transformed the vector into Agrobacterium LBA4404.Based on the highly efficient regeneration systerm which had been established previously,we optimized the regeneration system of recipient material,and transferred the DgDREB1A gene into chrysanthemum plants through Agrobacteriummediated transformations,and obtained 38 resistant plants.PCR analysis results showed that 8 plants of them were positive.We built the genetic transformation system of the cut chrysanthemum cv.‘Jinba’,and this will supply fundamental materials for the further selection.