温度与光照强度对烤烟番茄红素β-环化酶基因表达的影响

Effects of Temperature and Light Intensity on Expression of Lycopene Cyclase Gene in Tobacco(Nicotiana tabacum L.)

选用水培烤烟叶圆片,在3种温度、3种光照及其交互作用的正交试验处理下进行试验,检测了Lyc-β基因表达的结果表明:光照是影响Lyc-β基因表达的主要因子,其次为温度,最后是温度和光强交互作用。光照时间试验结果表明,Lyc-β基因的表达在光照处理6h时达到峰顶之后开始下调。

In this research,the tobacco leaf discs were treated with three different temperatures and three different light intensities using orthogonal experiment.The results of the expression of Lyc-β gene indicated that the key factor affecting the expression of Lyc-βwas light,followed by temperature,and then interactions of temperature with light.Moreover,the results of light experiment showed that the expression of Lyc-βincreased to the highest point after 6 hours’light-treatment,and then down-regulated.