慢性丙型肝炎患者外周血单个核细胞中APOBEC3GmRNA与血丙型肝炎病毒RNA的相关性

The correlation between APOBEC3G mRNA in peripheral blood mononuclear cells and serum hepatitis C viral RNA level

目的探讨慢性丙型肝炎患者外周血单个核细胞（PBMc）APOBEC3GmRNA表达水平与HCV慢性感染的关系。方法用TaqMan实时（RT）-PCR法检测49例慢性丙型肝炎患者以及31名健康人PBMC中APOBEC3GmRNA的水平，同时检测丙型肝炎患者外周血HCVRNA载量。所得数据采用SPSS11．0分析软件进行t检验和回归分析。结果慢性丙型肝炎患者PBMC中APOBEC3GmRNA的表达水平为（1．5±1．9）×10-5拷贝／mL，低于健康对照组（5．2±5．5）×10-5拷贝／mL，两组比较差异具有统计学意义（=-3．005，P〈0．01），而与外周血HCVRNA载量无明显相关性（r=-0．082，P〉O．05）。结论HCV感染抑制APOBEC3G的表达，但APOBEC3G不影响HCV复制。

Objective To study the relationship between APOBEC3G mRNA level in peripheral blood mononuclear cells （PBMC） and serum hepatitis C viral RNA level in patients with chronic hepatitis C infection. Methods TaqMan real-time fluorescence relative quantitative polymerase chain reaction （RT-PCR） was used to quantify APOBEC3G mRNA levels in PBMC from 49 patients with chronic hepatitis C （CHC） and 31 healthy subjects. The relationship between APOBEC3G mRNA level and hepatitis C virus （HCV） viral load was analyzed. SPSSll. 0 statistics software was used for t test and regression analysis. Results APOBEC3G mRNA level in CHC patients E（ 1.5 × 10-5 ±1.9 × 10-5） copy/roLl was significantly lower than that E（5.2 × 10-5± 5.5 × 10-5） copy/roLl in the healthy control subjects （t=-3. 005, P〈0. 01）. While APOBEC3G mRNA level was not related with HCV viral loads （ r =- 0. 082, P 〉 0. 05 ）. Conclusion HCV has an inhibitive effect on APOBEC3G expression, whereas APOBEC3G doesn＇t affect HCV replication directly in vivo.