金属螯合亲和膜吸附分离与纯化溶菌酶的研究(Ⅰ)──低温氧等离子体改性条件对亲和膜结构与吸附性能的影响

Studies on the Affinity Membrane for Adsorption and Purification of Lysozyme(Ⅰ) Influence of Low temperature Oxygen Plasma Treatment on Surface Structure and Adsorption Properties of the Affinity Membrane

用低温氧等离子体方法对聚丙烯微孔膜表面进行改性 .扫描电镜、红外光谱和光电子能谱综合分析结果表明 ,膜表面上产生了—OH、—COOH和 C O等极性基团 ,这些基团可被活化和偶联制亲和膜 .所制备的 Cu2 +离子金属螯合亲和膜用于对溶菌酶的吸附 ,在 2 0 min和 68W的最佳条件下 ,制成的亲和膜对溶菌酶的吸附量为 8μg/ cm2 .

In this paper, low temperature oxygen plasma method was used to modify the surface of PP(polypropylene) microporous membranes. The influence of oxygen plasma on the chemical structure and physical performance of the membrane was investigated with SEM, XPS and IR spectra. The results of XPS and IR showed that polar groups, —OH , —COOH , CO were created on these membrane surfaces. The groups —OH and —COOH were useful for activation and grafting process for affinity membrane with Cu 2+ as a metal chelating ligand. The influence of plasma treatment condition on the adsorbability of affinity membrane for lysozyme was carried out. The optimal adsorbability of lysozyme was 8 μg/cm 2 on the affinity membrane prepared for 20 min and under 68 W plasma condition. [WT5HZ]