Ⅲ型前列腺炎纳米细菌快速检测方法研究及临床评价

Rapid detection and clinical evaluation of nanobacteria infection in type Ⅲ prostatitis

目的通过对几种Ⅲ型前列腺炎纳米细菌感染检测方法评价比较,找出一种最快速、可靠的纳米细菌检测方法。方法以培养后间接免疫荧光鉴定结果为基准对照,分别采用间接免疫荧光法、茜素红钙染色法及PCR法对西南医院泌尿外科门诊150例Ⅲ型前列腺炎患者前列腺液标本进行纳米细菌检测,比较其阳性检出率、灵敏度和特异度差异。结果评价阳性检出率、灵敏度、特异度:间接免疫荧光法依次为23.3%、42.2%、100.0%;茜素红钙染色法依次为21.3%、34.9%、95.5%;PCR法依次为57.3%、95.2%、89.6%。间接免疫荧光法、茜素红钙染色法检出率与培养后间接免疫荧光法比较,均有统计学差异(P<0.05);而PCR法检出率与培养后间接免疫荧光法比较无统计学差异(P>0.05)。结论 PCR法检出率优于间接免疫荧光法及茜素红钙染色法,灵敏度及特异度均较高,可广泛应用于Ⅲ型前列腺炎患者纳米细菌感染快速检测。

Objective To discover the rapidest and most reliable method for detecting nanobacteria infection in type Ⅲ prostatitis by comparing several available methods.Methods Expressed prostatic secretion（EPS） specimens were obtained from 150 outpatients suffering from type Ⅲ prostatitis from the Department of Urology,Southwest Hospital,Chongqing,China.Nanobacteria infection in the EPS specimens was detected by indirect immunofluorescence staining（IIFS）,alizarin red staining of calcium,and polymerase chain reaction（PCR）.With the result of indirect immunofluorescence staining after culture（IIFSC） as a benchmark,the differences of the concerning the positive detection rate,sensitivity and specificity were compared.Results The positive detection rate,sensitivity and specificity of IIFS were 23.3%,42.2% and 100%,respectively.Those of alizarin red staining of calcium were 21.3%,34.9% and 95.5%,and those of PCR were 57.3%,95.2% and 89.6%,respectively.The positive detection rate of IIFS or alizarin red calcium staining had a statistical difference from that of IIFSC（P0.05）.However,there was no statistical difference between the positive detection rates of PCR and IIFSC（P0.05）.Conclusion PCR is superior to IIFS and alizarin red staining of calcium in terms of positive detection rate,and has high sensitivity and specificity as well.Therefore,PCR can be widely used for the rapid detection of nanobacteria infection in type Ⅲ prostatitis.