摘要
目的:体外研究蓝舌病毒湖北株3(BTV-HbC3)对小鼠前列腺癌细胞RM-1的感染性并探讨BTV-HbC3靶向性溶瘤的机制。方法:观察RM-1细胞感染BTV-HbC3的细胞病变效应;MTT法研究病毒致细胞病变率的特征;透射电镜观察感染病毒后细胞超微结构的变化;DNA Ladder分析病毒诱导细胞凋亡的情况;流式细胞仪测定病毒对RM-1细胞凋亡的影响。结果:BTV-HbC3感染RM-1细胞后有明显的细胞病变效应;DNA Ladder分析为阶梯状条带;透射电镜发现胞质内有大量病毒颗粒和典型细胞凋亡形态变化;流式细胞仪可见明显的细胞凋亡。结论:BTV-HbC3在体外能有效的感染RM-1细胞,并能诱导RM-1细胞凋亡。
Objective： To investigate the characteristics and the mechanism of bluetongue virus strain HbC3（HCMV） infecting mouse prostate cancer RM-1 cells in vitro.Methods： BTV-HbC3 was used to infect RM-1 cells,the the cytopathic effect（CPE） was observed,and the inhibition activity of RM-1 cell infected with BTV-HbC3 was determined by MTT.Transmission electron microscope（TEM） was adopted to study the changes of cell ultrastructure.DNA Ladder was taken to detect the apoptosis of RM-1 cells induced by BTV-HbC3.The apoptosis was detected by flow cytometry（FCM）.Results： RM-1 cells were sensitive to BTV-HbC3 infection,CPE was found in BTV-HbC3 infected RM-1 cells,and lots of virus particles were found in cytoplasm by TEM.Apoptotic cells were detected by FCM.Conclusion： BTV-HbC3 could infect RM-1 cells and replicate efficiently,and induce apoptosis in tumor cells.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2011年第2期176-179,195,共5页
Medical Journal of Wuhan University
