乳腺癌细胞Twist和Trkb的表达及其意义

Increased Expression of Twist and Trkb in Breast Cancer and Their Clinical Significance

目的:探讨乳腺癌细胞Twist和Trkb的表达及其意义。方法:培养人类乳腺癌细胞系MCF-7,并注射到裸鼠体内,3-4周后收集鼠移植瘤标本14例;同时收集了乳腺癌患者的标本86例。使用RT-PCR检测Twist和Trkb的表达,并回顾性分析二者与患者无疾病进展时间的关系。结果:在裸鼠体内,肺转移组织的Twist和Trkb高表达,表达产物的相对光密度值分别为2.1±0.2比1.4±0.2,2.3±0.4比1.5±0.3;t=2.4,2.8;P=0.03,0.02。乳腺癌患者Twist和Trkb表达明显高于正常乳腺组织(分别为1.67±0.32比0.83±0.23,1.58±0.41比0.65±0.24;t=2.6,P=0.02和t=3.01,P=0.01),乳腺癌转移淋巴结组织Twist和Trkb的表达明显高于原发灶(分别为2.38±0.22比1.84±0.43,2.17±0.41比1.62±0.32;t=3.23,P=0.01和t=2.84,P=0.03),乳腺癌患者Twist和Trkb表达与患者年龄、肿瘤大小无明显相关性(P>0.05),与淋巴结转移、临床分期和组织病理分级有关(P<0.05)。并且具有Trkb高表达患者的无疾病进展时间明显低于低表达患者[(28.4±2.2)比(32.7±2.4)月,LogRank=3.88,P=0.037]。但Twist高表达者与无疾病进展时间无明显关系[(29.9±2.6)比(31.5±2.5)月,LogRank=2.46,P=0.08]。结论:Twist和Trkb的高表达与乳腺癌的转移及发展呈正相关,高表达Trkb或许能预计患者的生存。

Objective： To study the expression of Twist and Trkb in human breast cancers,and to investigate the role of Twist and Trkb in breast cancer progression,metastasis,and prognosis.Methods： The MCF-7 human breast cancer cell line was cultured under mammosphere forming conditions and implanted into nude mice.RT-PCR technique was used to measure the expression of Twist and Trkb mRNA in the tissue samples from 14 nude mice and 86 human human breast tumors.Results： In the nude mice implanted with breast cancer cells,the level of the Twist and Trkb mRNA in the samples from metastasis tissues was higher than that of primary tissues（the relative OD value of Twist and Trkb mRNA was 2.1±0.2 vs 1.4±0.2,2.3±0.4 vs 1.5±0.3;t=2.4,2.8;P=0.03,0.02 respectively）.In human tumor samples,the level of the Twist and Trkb mRNA of lymph node metastasis was higher than that of breast tissues（1.67±0.32 vs 0.83±0.23,1.58±0.41 vs 0.65±0.24 respectively;t=2.6,P=0.02;t=3.01,P=0.01）.The level of the Twist and Trkb mRNA of breast cancers was higher than that of primary tissues（2.38±0.22 vs 1.84±0.43,2.17±0.41 vs 1.62±0.32 respectively;t=3.23,P=0.01;t=2.84,P=0.03）.The expression of Twist and Trkb was significantly correlated with clinical stages,tumor lymph node metastasis and loss of differentiation（all P0.05）.However,there was no correlation between Twist or Trkb mRNA levels and age or tumor size（all P0.05）.Increased expression of Twist correlated with shorter overall survival（[28.4±2.2] vs [32.7±2.4] months,LogRank=3.88,P=0.037）,while no significant correlations were observed between increased expression of Trkb and shorter overall survival（[29.9±2.6] vs [31.5±2.5] months,LogRank=2.46,P=0.08）.Conclusion： Expression of Twist and Trkb associate with breast cancer infiltration and metastasis,and increased expression of Twist may implicate in breast cancer prognosis.