环氧化酶选择性抑制剂NS-398对Hela细胞线粒体膜电位改变及诱导凋亡作用研究

Effects of NS-398 on the apoptosis and mitochondrial membrane potential changes of Hela cells

目的探讨环氧化酶-2(COX 2)选择性抑制剂NS-398诱导宫颈癌细胞凋亡的作用机制。方法用不同浓度NS-398作用于人宫颈癌Hela细胞,CCK-8法检测NS-398对宫颈癌细胞增殖的影响,应用罗丹明(Rhodanmine)123染色检测癌细胞线粒体膜电位改变;琼脂糖凝胶电泳检测DNA降解梯度(ladder)。结果 NS-398对Hela细胞的增殖具有明显抑制作用;其抑制率随药物浓度的增高、药物作用时间的延长而显著增高。NS-398作用24 h可以使线粒体膜电位显著降低且与NS-398浓度密切相关;作用48 h可以诱导Hela细胞发生凋亡。结论 NS-398对宫颈癌Hela细胞生长具有显著的抑制作用。其可能通过线粒体途径诱导癌细胞凋亡。

Objective： To investigate the effects of COX-2 selective inhibitor NS-398 on a human cervical carcinoma cell line, Hela, and explore the possibility of its application in anticancer treatment. Methods： Human cervical carcinoma Hela cells were treated with different concentrations of NS-398. The inhibitory effect of NS-398 on proliferation of Hela cells was assessed by Cell Counting Kit-8 （CCK-8） method. Rhodanmine 123 staining was applied to detect mitochondrial membrane potential changes in the cells, and agarose gel electrophoresis was used to show DNA ladders. Results： NS-398 significantly inhibited the growth of Hela cells. The inhibition rate increased as the concentration and treatment time increased. The mitochondrial membrane potential reduced significantly when Hela cells were treated with NS-398 for 24 hours and this effect was closely related to the concentration of NS 398. Apoptosis was observed in Hela cells treated with NS-398 for 48 hours. Conclusions： NS-398 significantly inhibited the growth of cervical carcinoma cells and may induce the apoptosis of the cells through a mitochondrial pathway.