递质氨基酸的毛细管电泳-安培检测

Determination of Amino Acids in Transmitters by Capillary Electrophoresis-Amperometric Detection with Carbon Fiber Microelectrode

研究了毛细管电泳安培检测氨基酸的方法。氨基酸用萘 -2,3 -二羧醛 (NDA)衍生为电活性物质。碳纤维电极采用1.5V ,30s固定电压电化学预处理 ,检测结果令人满意。作者还对最佳衍生反应条件 ,电极预处理条件对氨基酸衍生物电化学行为的影响进行了研究。该法对谷氨酸、甘氨酸、丙氨酸、γ -氨基丁酸的检出限分别为2.5×10-7、2.5×10-7、5.0×10-8、2.5×10-7 mol/L。

A method for the determination of amino acids in transmitters by electrophoresis-amperometric detector with carbon fiber microelectrode pretreated by anodization at 1.5 V(vs. SCE) for 30 s is presented. The amino acids were derivatized with naphthalene _ 2,3 _ dicarboxyaldehyde (NDA)to produce electroactive substances. The derivatization reaction conditions and electrochemical regime were optimized. The detection limits of the amino acids are 2.5×10-7, 2.5×10-7, 5.0×10-8, 2.5×10-7 mol/L for glutamic acid, glycine, β _ alanine and γ_ aminobutyric acid, respectively.