喉癌组织中VEGF-D表达与淋巴管生成及预后的关系

The relationship between vascular endothelial growth factor D expression and lymphangiogenesis as well as prognosis in human laryngeal carcinoma

目的探讨血管内皮生长因子-D(Vessel endothelium growth factor D,VEGF-D)在喉癌组织中的表达规律及与淋巴管密度(LVD)和淋巴转移之间的关系及其在喉癌预后中的意义。方法以45例经病理确诊的喉癌组织为实验组,以20例喉良性病变组织为对照组,采用免疫组化SP法对上述组织中VEGF-D蛋白的表达进行分析,5'-核苷酸酶-碱性磷酸酶双重染色法(5'-Nase-ALP)计数淋巴管密度(LVD),并结合临床病理特征和生存资料进行相关分析。结果在喉癌组织中VEGF-D的阳性率为42.2%,明显高于在良性病变组织的表达水平(<0.05)。VEGF-D表达与瘤内和瘤周LVD、淋巴结转移、淋巴管浸润显著相关(<0.01),与年龄、性别、肿瘤部位及组织学分级无关(>0.05)。Kaplan-Meier生存曲线分析表明VEGF-D的表达与生存率呈负相关(<0.05)。结论 VEGF-D促进喉癌淋巴管生成和淋巴管转移,可以成为喉癌评估预后的重要分子指标。

Objective To investigate the expression of vascular endothelial growth factor D（VEGF-D） in human laryngeal cancer to explore the relationship of the expression with lymphangiogenesis,lymph node metastasis and their prognosis significance. Methods Forty five specimens of human laryngeal cancer and twenty benign laryngeal tissues were used to observe the expression of VEGF-D by immunohistochemistry S-P method. The lymphatic vessels density（LVD） was evaluated by 5＇-Nucleotides-alkaline phosphates double staining,then the role of VEGF-D in laryngeal cancer prognosis was judged by Kaplan-meier method. Results The positive rate of VEGF-D expression is 42.2% in 45 laryngeal cancer samples,which is much higher in tumor tissue than in the normal tissue（ 0.05） . The expression of VEGF-D was significantly correlated with peritumoral LVD,intratumoral LVD,lymph nodes metastasis and lymphovascular invasion（ 0.01） ,but with no significant association with their age,gender,histological stage,location and TNM stage（ 0.05） . Kaplan-meier test indicated that the expression of VEGF-D was positively correlated with survival rate（ 0.05） . Conclusion The expression of VEGF-D protein was correlated with lymphangiogenesis and lymphatic metastasis,which may be helpful for judging prognosis in laryngeal cancer treatment.