人神经胶质祖细胞移植于小鼠脑内两侧侧脑室周区后发育成人星形胶质细胞

Differentiation of human glia progenitor cells into astrocytes after their transplantation into bilateral periarea of lateral ventricles of mouse brain

目的观察人神经胶质祖细胞移植于小鼠脑内两侧侧脑室周区后的形态结构发育变化。方法将人神经胶质祖细胞移植入出生1天的小鼠脑内两侧侧脑室周区,用免疫荧光组化技术和共聚焦显微镜观察移植6个月后,小鼠脑内人神经胶质祖细胞的分布、分化和形态结构的特点。结果人神经胶质祖细胞移植6个月后成功地在小鼠脑内存活并广泛分布,主要发育成人神经胶质酸性蛋白(glial fibrillary acidic protein GFAP)阳性的星形胶质细胞,表现人星形胶质细胞的形态结构特点,部分神经胶质祖细胞分化成硫酸软骨素蛋白多糖(chondroitin sulphate proteoglycan,NG2)阳性的细胞。结论人神经胶质祖细胞移植小鼠脑内环境中主要分化为GFAP阳性的星形胶质细胞和部分NG2阳性细胞。

Objective To observe the mophological development and differentiation of human gila progenitor cells after their transplantation into bilateral periarea of the lateral ventricles of mouse brain. Method Human glia progenitor cells were transplanted into bilateral periarea of the lateral ventricles of mouse brain at first postnatal day（PND1） ,6 months after transplantation,the distribution,differentiation and mophological features of the human cells were observed by immuno fluorescence staining and confocal microscope. Results Human glia progenitor cells were survived and distributed widely in mouse brain,differentiated into glial fibrillary acidic protein（GFAP） positive astrocytes mainly with mophological characteristics of human astrocytes and chondroitin sulphate proteoglycan（NG2） positive cells. Conclusion Human glia progenitor cells were differentiated into astrocytes mainly and some NG2 positive cells after transplantation into mouse brain.