硝普钠促进静脉移植BMSCs向溃疡性结肠炎大鼠结肠的迁移

Sodium nitroprusside promoting the migration of vein transplanted bone marrow mesenchymal stem cells to the colon of ulcerative colitis rat

目的:观察硝普钠能否促进静脉移植的大鼠骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)向溃疡性结肠炎(Ulcerativecolitis,UC)大鼠结肠的迁移。方法:分离培养4、’,6-二脒基-2-苯基吲哚盐酸(4’,6-diamidino-2-phenylindole,DAPI)荧光标记SD大鼠BMSCs。免疫复合法建立UC大鼠模型,1、2组UC大鼠均经尾静脉注射DAPI标记BMSCs的细胞悬液,2组细胞悬液还含0.2 mg/kg体重的硝普钠。移植后第7、14天各处死5只大鼠,取溃疡及非溃疡部位结肠做恒冷切片,荧光显微镜下分别计数各组DAPI标记BMSCs在溃疡及非溃疡部位结肠的细胞数。结果:移植第7、14天,2组结肠溃疡部位阳性细胞数均明显高于非溃疡部位(P<0.01);移植第7天,2组溃疡及非溃疡部位的阳性细胞数均高于1组(P<0.05)。结论:静脉移植BMSCs的同时给予硝普钠能促进BMSCs向结肠溃疡部位的迁移。

Objective：To investigate the effect of Sodium nitroprusside（SNP） in promoting the migration of vein transplanted bone marrow mesenchymal stem cells（BMSCs）to the colon of ulcerative colitis rat.Methods：BMSCs of SD rat were isolated,cultured and marked with DAPI.Ulcerative colitis（UC） rat was induced with immune-combined TNBS/ethanol.UC rats in group 1 and 2 was received caudal vein injection of DAPI labeled BMSCs cell suspension;but 0.2 mg Sodium nitroprusside/kg body weight was also injected in group 2.5 rats in each group were sacrificed on day 7 and 14 after injection.The colon of ulcerative or non-ulcerative parts were prepared as cryostat sections.The numbers of BMSCs both in colon of ulcerative or non-ulcerative parts were counted under fluorescent microscope respectively.Results：On the 7th and 14th day after injection,the number of marked BMSCs in the colonic ulcerative part was more than that in non-ulcerative parts（P 0.01） both in group 1 and 2;On the 7th day,the marked BMSCs number of group 2 both in the colon of ulcerative or non-ulcerative parts were higher than those in group 1（P0.05）.Conclusion：SNP could encourage vein transplanted BMSCs migranting to the injured colonic tissue by the way that vein transplantion of BMSCs combined supply of SNP at the same time.